Cell No. : Cell Name
RCB5378 : P3-X63-Ag8-U/hEphB6-T49-25
update : 2024/09/04
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| Comment | |
| Comment from the depositor | Mouse hybridoma cell line producing anti-human Ehph B6 monoclonal antibody. |
| Terms and conditions | 1) The use of the BIOLOGICAL RESOURCE need sapre-approval letter by the developer, Dr.Toshimitsu Matsui (matsui.kobe@gmail.com). 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Growth Factors. 2000;18(1):63-78) designated by the DEPOSITOR is required.
|
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KATAYAMA, Yoshio
|
| Originator |
MATSUI, Toshimitsu
|
| Year of deposit |
2021
|
| Original cell |
anti-hEphB6-ECD3/P3X63Ag8U1
|
| Cloning (depositor) |
Yes
|
| _Date (depositor) |
1997
|
| Animal |
_mouse x mouse
< Hybrid
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A6ZU
|
|
hybridoma
|
Fusion_cell_1 name |
P3-X63-Ag8-U
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen
|
| Fusion_cell_2 strain |
mouse, Balb/c
|
| Fusion_cell_2 gender |
Unknown
|
|
Fusion method |
50% PEG
|
|
Antibody name |
hEphB6-T49-25
|
| Subclass |
IgG1
|
| Immunogen |
GST-fusion extracellular domain region 3 of human EphB6 recombinant protein
|
|
Antigen_name |
human EphB6
|
| Antigen_m.w. |
135k-Da
|
| Antigen_character |
Type I tyrosine kinase receptor
|
| Antigen_epitope |
human EphB6 extracellular domain region 3 (298aa~424aa)
|
|
Antigen_positive |
T cell / T cell tumor cell line・cerebrum / thymus
|
| Antigen_negative |
B cell
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI-1640+10%FCS
|
RPMI1640 + 10% FBS
|
| Antibiotics |
penicillin, kanamycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 4-10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Antibody subclass |
|
IgG1(Kappa)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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