Cell No. : Cell Name RCB5315 : 53BP1,BRCA1-TK6  update : 2025/11/14
Comment
Comment from the depositor			TK6 cells are one of the most commonly used human B lymphocyte-derived cell for cancer research. 53BP1, BRCA1- disrupted TK6 cells.
Terms and conditions			1)The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 2018 115(45):E10642-E10651) designated by the DEPOSITOR is requested.
Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form			Order Form(C-0005.pdf)   MTA(C-0065.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	IPR	Kyoto University (Japan)
		Depositor	TAKEDA, Shunichi & SASANUMA, Hiroyuki
		Originator	TAKEDA, Shunichi & SASANUMA, Hiroyuki
		Year of deposit	2020
		Another name	53BP1, BRCA1-/-
		Original cell	TK6
		Cloning (depositor)	Yes
		_Date (depositor)	2019
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	5 years
		Tissue	spleen
		Disease name	Hereditary spherocytosis
		Classification	transformed
		Recombinant	recombinant
		Genome Editing	53BP1 gene, BRCA1 gene
		Vector	DT-ApA/MARKERR
		Recombinant virus	No
		Lifespan	infinite
		Morphology	lymphocyte-like
		Contact inhibition	No
		Tumorigenesis	No
			deposit info	lot info
		Medium	Medium List
		Culture type	Suspension cells	Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium	RPMI1640, Horse serum 10%, Sodium pyruvate 0.2mg/ml	RPMI1640 + 10% HS + 0.2mg/ml Sodium pyruvate
		Antibiotics	Penicillin / Streptomycin	Free
		Passage method		dilution
	Culture information	Passage cell No	> 0.1x10 6 cells/ml	1x10 5/mL
		SC frequency		3 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	Cell Freezing Reagents	CELLBANKER 1
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Mycoplasma	(-)
		Animal PCR		OK
		Chromosome mode	Trisomy 13
		Saturation density	1x10 6 cells/ml
		Doubling time	13 hr
		Plating efficiency	70 %
		STR(human)		OK
Reference information		Reference	6
		User's Publication	0

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Reference

17853  Zong D, Adam S, Wang Y, Sasanuma H, Callén E, Murga M, Day A, Kruhlak MJ, Wong N, Munro M, Ray Chaudhuri A, Karim B, Xia B, Takeda S, Johnson N, Durocher D, Nussenzweig A.  BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation  Mol Cell  2019  73(6):1267-1281.e7  PubMed ID: 30704900   DOI: 10.1016/j.molcel.2018.12.010

17846  Sasanuma H, Tsuda M, Morimoto S, Saha LK, Rahman MM, Kiyooka Y, Fujiike H, Cherniack AD, Itou J, Callen Moreu E, Toi M, Nakada S, Tanaka H, Tsutsui K, Yamada S, Nussenzweig A, Takeda S.  BRCA1 ensures genome integrity by eliminating estrogen-induced pathological topoisomerase II-DNA complexes  Proc Natl Acad Sci U S A  2018  115(45):E10642-E10651  PubMed ID: 30352856   DOI: 10.1073/pnas.1803177115

2127  Gupta RS.  Diphtheria toxin resistance in human lymphoblast lines  Biochem Biophys Res Commun  1980  94(4):1303-10  PubMed ID: 7396963   DOI: 10.1016/0006-291x(80)90561-6

279  Skopek TR, Liber HL, Penman BW, Thilly WG.  Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay  Biochem Biophys Res Commun  1978  84(2):411-6  PubMed ID: 214074   DOI: 10.1016/0006-291x(78)90185-7

281  Levy JA, Buell DN, Creech C, Hirshaut Y, Silverberg H.  Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines  J Natl Cancer Inst  1971  46(3):647-54  PubMed ID: 4323138

280  Levy JA, Virolainen M, Defendi V.  Human lymphoblastoid lines from lymph node and spleen  Cancer  1968  22(3):517-24  PubMed ID: 5673231   DOI: 10.1002/1097-0142(196809)22:3<517::aid-cncr2820220305>3.0.co;2-a

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