Cell No. : Cell Name
RCB5305 : NP-2/CD4/EcoVR
update : 2024/01/17
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| Comment | |
| Comment from the depositor | NP-2 is derived from a human glioma cell line. A CD4 and an ecotropic mouse retrovirus receptor (EcoVR) gene are transduced to NP-2 cells. This NP-2/CD4/EcoVR (NP-2/CD4) is resistant to the HIV strains tested. When transduced several HIV co-receptors using an ecotropic mouse retrovirus vector, this cell line become susceptible to HIVs. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Biochem Biophys Res Commun 1999 258(2):313-21) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
OUE, Atsushi
|
| Originator |
HOSHINO, Hiroo
|
| Year of deposit |
2020
|
| Original cell |
NP 2
|
| Cloning (depositor) |
Yes
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
63 years
|
| Tissue |
frontal lobe, left
|
| Disease name |
anaplastic glioma
|
| Classification |
cancer
|
| Recombinant |
recombinant
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| Exogene |
human CD4, mouse EcoVR,
|
| Vector |
pBabe-hygro
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| Selection |
G418 (500μg/ml), Hygromycin (50 μg/ml)
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_1R18
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
MEM + 10% (v/v) heat-inactivated fetal bovine serum
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MEM + 10% FBS (heat inactivated)
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| Antibiotics |
Penicillin G (100units/ml), Streptomycin (100μg/ml)
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Free
|
|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage ratio |
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1 : 4-8 split
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| SC frequency |
Subculture : 2 times/week
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
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| Animal PCR |
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OK
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| STR(human) |
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OK
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| Reference information |
Reference |
1
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| User's Publication |
0
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