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Cell No. : Cell Name
RCB5304 : 40LB  update : 2024/02/16
Comment
Comment from the depositorA fibroblast cell line, BALB/3T3 A31, stably transfected with expression vectors encoding mouse CD40-ligand (CD40L) and BAFF. Used as feeder cells for B cell culture, they support efficient proliferation and survival of B cells.
Terms and conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nojima et al. Nat Commun 2011 2:465) designated by the DEPOSITOR is requested. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Remarks
approver's address
×
English
Address
Tokyo University of Science, Research Institute for Biomedical Science
2669 Yamazaki, Noda City Chiba, 278-0022, Japan
Dr.KITAMURA Daisuke
Fax. +81-4-7121-4071
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KITAMURA, Daisuke
Originator KITAMURA, Daisuke
Year of deposit 2020
Another name 40LB-NB
Original cell BALB/3T3 A31
Cloning (depositor) No
Animal _mouse < Mammals
Strain name BALB/c
Genus Mus
Species musculus
Gender Unknown
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene mouse CD40L cDNA in pApuro2, mouse BAFF cDNA in pCA-neo
Vector pApuro2, pCA-neo
Selection puromycin (2.5 μg/ml), G418 (0.5 mg/ml)
Recombinant virus No
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_A6ZS
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM (high glucose, WAKO #044-29765) + 10% FBS (heat-inactivated) DMEM(high glucose,without sodium pyruvate) + 10% FBS (heat inactivated)
Antibiotics 100 U/ml penicillin, 100 μg/ml streptomycin Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10 6 /φ10 cm dish
Passage ratio 1 : 6 split
SC frequency Passage the cells every 3 days with 6-fold dilutions Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium DMEM + 20% FBS + 10% DMSO DMEM(high glucose,without sodium pyruvate) + 20% FBS (heat inactivated) + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
10574  Kei Haniuda, Daisuke Kitamura  Induced Germinal Center B Cell Culture System  Bio-protocol  2019  9(4):e3163  PubMed ID: 33654969   DOI: 10.21769/BioProtoc.3163
10573  Takuya Nojima, Kei Haniuda, Tatsuya Moutai, Moeko Matsudaira, Sho Mizokawa, Ikuo Shiratori, Takachika Azuma, Daisuke Kitamura  In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo  Nat Commun  2011  2:465  PubMed ID: 21897376   DOI: 10.1038/ncomms1475

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