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Cell No. : Cell Name
RCB5304 : 40LB update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | A fibroblast cell line, BALB/3T3 A31, stably transfected with expression vectors encoding mouse CD40-ligand (CD40L) and BAFF. Used as feeder cells for B cell culture, they support efficient proliferation and survival of B cells. | |||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nojima et al. Nat Commun 2011 2:465) designated by the DEPOSITOR is requested. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | KITAMURA, Daisuke | ||
| Originator | KITAMURA, Daisuke | |||
| Year of deposit | 2020 | |||
| Another name | 40LB-NB | |||
| Original cell | BALB/3T3 A31 | |||
| Cloning (depositor) | No | |||
| Animal | _mouse < Mammals | |||
| Strain name | BALB/c | |||
| Genus | Mus | |||
| Species | musculus | |||
| Gender | Unknown | |||
| Tissue | embryo | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | mouse CD40L cDNA in pApuro2, mouse BAFF cDNA in pCA-neo | |||
| Vector | pApuro2, pCA-neo | |||
| Selection | puromycin (2.5 μg/ml), G418 (0.5 mg/ml) | |||
| Recombinant virus | No | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_A6ZS | |||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | DMEM (high glucose, WAKO #044-29765) + 10% FBS (heat-inactivated) | DMEM(high glucose,without sodium pyruvate) + 10% FBS (heat inactivated) | ||
| Antibiotics | 100 U/ml penicillin, 100 μg/ml streptomycin | Free | Passage method | 0.25% Trypsin | Culture information | Passage cell No | 3 x 10 6 /φ10 cm dish |
| Passage ratio | 1 : 6 split | |||
| SC frequency | Passage the cells every 3 days with 6-fold dilutions | Subculture : 2 times/week | ||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | DMEM + 20% FBS + 10% DMSO | DMEM(high glucose,without sodium pyruvate) + 20% FBS (heat inactivated) + 10% DMSO | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Images | ||||
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| Reference information | Reference | 2 | ||
| User's Publication | 1 | |||
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