Cell No. : Cell Name
RCB5296 : RMM-CAF
update : 2025/03/26
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| Comment | |
| Comment from the depositor | RMM-CAF is a non-neoplastic cloned cell line isolated from a rat malignant amelanotic melanoma cell strain RMM-C (RCB5294). RMM-CAF is useful for studies on characterization of tumor stromal cells, particularly in the mutual association with a neoplastic cell line RMM-A1 (RCB5295) that was isolated simultaneously from RMM-C. |
| Terms and conditions | １) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Histol Histopathol 2016 31(11):1195-207) designated by the DEPOSITOR is requested. ２) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Yamate, Jyoji
|
| Originator |
Yamate, Jyoji
|
| Year of deposit |
2020
|
| Cloning (depositor) |
Yes
|
| _Date (depositor) |
2016
|
| _Method (depositor) |
dilution cloning, twice
|
| Animal |
_rat
< Mammals
|
| Strain name |
F344
|
| Gender |
Male
|
| Age at sampling |
24 month
|
| Tissue |
skin (auricle)
|
| Primary focus |
|
| Disease name |
amelanotic melanoma
|
| Classification |
other
|
| Recombinant |
non-recombinant
|
| Year of origin |
2013
|
|
Lifespan |
infinite
|
| Morphology |
other
(mesenchymal cell-like)
|
| Contact inhibition |
Yes
|
| Tumorigenesis |
No
|
| Cellosaurus(Expasy) |
CVCL_A6UW
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM + 10% FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
Penicillin Streptomycin 1%
|
Free
|
|
Passage method |
0.1% Trypsin
|
0.1% Trypsin 0.02% EDTA
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Culture information
|
Passage ratio |
|
1 : 5 split
|
| SC frequency |
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
Slow freezing
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Chromosome mode |
66-70
|
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
5536
A Bondoc , H M Golbar , M Pervin, C Katou-Ichikawa, M Tanaka, T Izawa, M Kuwamura, J Yamate
Participation of Tumor-Associated Myeloid Cells in Progression of Amelanotic Melanoma (RMM Tumor Line) in F344 Rats, with Particular Reference to MHC Class II- and CD163-Expressing Cells
Cancer Microenviron
2017
10(1-3):9-24
PubMed ID: 28623530
DOI: 10.1007/s12307-017-0193-x
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|
4438
Alexandra Bondoc, Chisa Katou-Ichikawa, Hossain M Golbar, Miyuu Tanaka, Takeshi Izawa, Mitsuru Kuwamura , Jyoji Yamate
Establishment and characterization of a transplantable tumor line (RMM) and cell line (RMM-C) from a malignant amelanotic melanoma in the F344 rat, with particular reference to galectin-3 expression in vivo and in vitro
Histol Histopathol
2016
31(11):1195-207
PubMed ID: 26949998
DOI: 10.14670/HH-11-748
|
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