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Cell No. : Cell Name
RCB5293 : MEF_Hsp47 KO-13  update : 2024/01/17
Comment
Comment from the depositorMouse embryonic fibroblasts derived from Hsp47-deficient mouse.
Terms and conditions1) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nagai et al. J Cell Biol 2000 150(6):1499-506) designated by the DEPOSITOR is requested. 3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
approver's address
×
English
Address
Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555 Japan
Dr.Nagata Kazuhiro
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor MAGATA, Kazuhiro
Originator MAGATA, Kazuhiro
Year of deposit 2020
Another name Hsp47 (－/－)
Cloning (depositor) No
Animal _mouse < Mammals
Strain name C57BL/6J x 129/Ola
Genus Mus
Species musculus
Gender Unknown
Age at sampling embryo
Tissue whole
Classification mutant
Recombinant recombinant
Exogene Hsp47-deficient
Vector pMCDT-A
Recombinant virus No
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_A7BE
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM (high glucose) + 10% FBS DMEM (high glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin 0.25% Trypsin
Culture information Passage ratio 1 : 5 split 1 : 4-6 split
SC frequency Subculture : once/3 days Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER or Cell Reservoir One CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
13677  Yoshihito Ishida, Akitsugu Yamamoto, Akira Kitamura, Shireen R Lamandé, Tamotsu Yoshimori, John F Bateman, Hiroshi Kubota, Kazuhiro Nagata  Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection  Mol Biol Cell  2009  20(11):2744-54  PubMed ID: 19357194   DOI: 10.1091/mbc.e08-11-1092
12347  Yoshihito Ishida, Hiroshi Kubota, Akitsugu Yamamoto, Akira Kitamura, Hans Peter Bächinger, Kazuhiro Nagata  Type I collagen in Hsp47-null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis  Mol Biol Cell  2006  17(5):2346-55  PubMed ID: 16525016   DOI: 10.1091/mbc.e05-11-1065
12346  N Nagai, M Hosokawa, S Itohara, E Adachi, T Matsushita, N Hosokawa, K Nagata  Embryonic lethality of molecular chaperone hsp47 knockout mice is associated with defects in collagen biosynthesis  J Cell Biol  2000  150(6):1499-506  PubMed ID: 10995453   DOI: 10.1083/jcb.150.6.1499

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