Cell No. : Cell Name RCB5265 : ATL-5S  update : 2024/04/19
Comment
Comment from the depositor			ATL-5S is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-5S cells are positive for HTLV-1 antigens.
Terms and conditions			In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	OUE, Atsushi
		Originator	HOSHINO, Hiroo
		Year of deposit	2019
		Animal	_human < Mammals
		Strain name	Homo
		Genus	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	34 years
		Tissue	blood
		Disease name	adult T-cell leukemia (ATL)
		Classification	cancer
		Lifespan	infinite
		Morphology	lymphocyte-like
		Surface antigen (depositor)	CD4(+), CD3(-), CD8(-)
		BSL	BSL2
		Cellosaurus(Expasy)	CVCL_A410
			deposit info	lot info
		Medium	Medium List
		Culture type	Suspension cells	Suspension cells
		Culture medium	RPMI-1640 + 20% heat-inactivated fetal calf serum	RPMI1640 + 20% FBS (heat inactivated)
		Culture method
		Antibiotics	100units/ml Penicillin G、100μg/ml Streptomycin	Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 3-6 split
		SC frequency	Subculture: 2times/week	Subculture : 1-2 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	Medium + 10% DMSO	Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Virus (HIV)		Undetected
		Virus (HTLV-1)	(+)	DNA (+)
		Virus (EBV)		(-)
		STR(human)		OK
		deposit info
		lot info	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 9,13 12,13 11,12 9,11 14,17 8,9 X,Y 8,11 10
Reference information		Reference	6
		User's Publication	0

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Reference

3853  Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.  Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells  Jpn J Cancer Res  1987  78(10):1112-20  PubMed ID: 2890614

2857  Shimotohno K, Miwa M, Slamon DJ, Chen IS, Hoshino H, Takano M, Fujino M, Sugimura T.  Identification of new gene products coded from X regions of human T-cell leukemia viruses  Proc Natl Acad Sci U S A  1985  82(2):302-6  PubMed ID: 2982148   DOI: 10.1073/pnas.82.2.302

2859  Hoshino H, Tanaka H, Shimotohno K, Miwa M, Akatsuka T, Odaka T.  Transmission of human T-cell leukemia virus type I to an S+L- cat kidney cell line  Virology  1985  147(1):223-6  PubMed ID: 2998067   DOI: 10.1016/0042-6822(85)90244-2

3903  Miwa M, Shimotohno K, Hoshino H, F  Detection of pX proteins in human T-cell leukemia virus (HTLV)-infected cells by using antibody against peptide deduced from sequences of X-IV DNA of HTLV-I and Xc DNA of HTLV-II proviruses  Gan  1984  75(9):752-5  PubMed ID: 6094296

3521  H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura  Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia  Proc Natl Acad Sci U S A  1983  80(19):6061-5  PubMed ID: 6193528   DOI: 10.1073/pnas.80.19.6061

3912  Hoshino H, Shimoyama M, Miwa M, Sugimura T.  Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay  Proc Natl Acad Sci U S A  1983  80(23):7337-41  PubMed ID: 6316359   DOI: 10.1073/pnas.80.23.7337

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