Cell No. : Cell Name
RCB5265 : ATL-5S
update : 2024/11/11
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| Comment | We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2. |
| Comment from the depositor | ATL-5S is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-5S cells are positive for HTLV-1 antigens. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
OUE, Atsushi
|
| Originator |
HOSHINO, Hiroo
|
| Year of deposit |
2019
|
| Animal |
_human
< Mammals
|
| Strain name |
Homo
|
| Genus |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
34 years
|
| Tissue |
blood
|
| Disease name |
adult T-cell leukemia (ATL)
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Surface antigen (depositor) |
CD4(+), CD3(-), CD8(-)
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_A410
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI-1640 + 20% heat-inactivated fetal calf serum
|
RPMI1640 + 20% FBS (heat inactivated)
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| Antibiotics |
100units/ml Penicillin G、100μg/ml Streptomycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 3-6 split
|
| SC frequency |
Subculture: 2times/week
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
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OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
(+)
|
DNA (+)
|
| Virus (EBV) |
|
(-)
|
| STR(human) |
|
OK
|
|
deposit info
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
9,13
|
12,13
|
11,12
|
9,11
|
14,17
|
8,9
|
X,Y
|
8,11
|
10
|
|
| Reference information |
Reference |
6
|
| User's Publication |
0
|
| Reference |
|
3853
Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.
Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells
Jpn J Cancer Res
1987
78(10):1112-20
PubMed ID: 2890614
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2857
Shimotohno K, Miwa M, Slamon DJ, Chen IS, Hoshino H, Takano M, Fujino M, Sugimura T.
Identification of new gene products coded from X regions of human T-cell leukemia viruses
Proc Natl Acad Sci U S A
1985
82(2):302-6
PubMed ID: 2982148
DOI: 10.1073/pnas.82.2.302
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2859
Hoshino H, Tanaka H, Shimotohno K, Miwa M, Akatsuka T, Odaka T.
Transmission of human T-cell leukemia virus type I to an S+L- cat kidney cell line
Virology
1985
147(1):223-6
PubMed ID: 2998067
DOI: 10.1016/0042-6822(85)90244-2
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3903
Miwa M, Shimotohno K, Hoshino H, F
Detection of pX proteins in human T-cell leukemia virus (HTLV)-infected cells by using antibody against peptide deduced from sequences of X-IV DNA of HTLV-I and Xc DNA of HTLV-II proviruses
Gan
1984
75(9):752-5
PubMed ID: 6094296
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3521
H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura
Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia
Proc Natl Acad Sci U S A
1983
80(19):6061-5
PubMed ID: 6193528
DOI: 10.1073/pnas.80.19.6061
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3912
Hoshino H, Shimoyama M, Miwa M, Sugimura T.
Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay
Proc Natl Acad Sci U S A
1983
80(23):7337-41
PubMed ID: 6316359
DOI: 10.1073/pnas.80.23.7337
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