Cell No. : Cell Name RCB5264 : ATL-3I  update : 2025/03/25
Comment			BSL2. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2.
Comment from the depositor			ATL-3I is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-3I cells are positive for HTLV-1 antigens.
Terms and conditions			In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	OUE, Atsushi
		Originator	HOSHINO, Hiroo
		Year of deposit	2019
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	71 years
		Tissue	blood
		Disease name	adult T-cell leukemia (ATL)
		Classification	cancer
		Lifespan	infinite
		Morphology	lymphocyte-like
		Surface antigen (depositor)	CD4(+), CD3(-), CD8(-)
		BSL	BSL2
		Cellosaurus(Expasy)	CVCL_A408
			deposit info	lot info
		Medium	Medium List
		Culture type	Suspension cells	Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium	RPMI-1640 + 20% heat-inactivated fetal calf serum	RPMI1640 + 20% FBS (heat inactivated)
		Antibiotics	100units/ml Penicillin G、100μg/ml Streptomycin	Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 2-4 split
		SC frequency	Subculture: 2times/week	Subculture: 2times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	Medium + 10% DMSO	Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Virus (HIV)		Undetected
		Virus (HTLV-1)		DNA (+)
		Virus (EBV)		(-)
		STR(human)		OK
Reference information		Reference	5
		User's Publication	0

Top

Reference

2443  Seki J, Ikeda R, Hoshino H.  Dimethyl sulfoxide and related polar compounds enhance infection of human T cells with HIV-1 in vitro  Biochem Biophys Res Commun  1996  227(3):724-9  PubMed ID: 8886001   DOI: 10.1006/bbrc.1996.1576

3853  Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.  Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells  Jpn J Cancer Res  1987  78(10):1112-20  PubMed ID: 2890614

2609  Hoshino H, Shimizu N, Shimada N, Takita T, Takeuchi T.  Inhibition of infectivity of human immunodeficiency virus by oxetanocin  J Antibiot (Tokyo)  1987  40(7):1077-8  PubMed ID: 3650259   DOI: 10.7164/antibiotics.40.1077

3521  H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura  Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia  Proc Natl Acad Sci U S A  1983  80(19):6061-5  PubMed ID: 6193528   DOI: 10.1073/pnas.80.19.6061

3912  Hoshino H, Shimoyama M, Miwa M, Sugimura T.  Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay  Proc Natl Acad Sci U S A  1983  80(23):7337-41  PubMed ID: 6316359   DOI: 10.1073/pnas.80.23.7337

Top

User's Publication