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Cell No. : Cell Name
RCB5264 : ATL-3I  update : 2024/11/11
CommentBSL2. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2.
Comment from the depositorATL-3I is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-3I cells are positive for HTLV-1 antigens.
Terms and conditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OUE, Atsushi
Originator HOSHINO, Hiroo
Year of deposit 2019
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 71 years
Tissue blood
Disease name adult T-cell leukemia (ATL)
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Surface antigen (depositor) CD4(+), CD3(-), CD8(-)
BSL BSL2
Cellosaurus(Expasy) CVCL_A408
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI-1640 + 20% heat-inactivated fetal calf serum RPMI1640 + 20% FBS (heat inactivated)
Antibiotics 100units/ml Penicillin G、100μg/ml Streptomycin Free
Passage method dilution
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture: 2times/week Subculture : 1-2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) DNA (+)
Virus (EBV) (-)
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11 8,9 9,11 9,12 16 6,9 X,Y 8,9 12,13
Reference information Reference 5
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Reference
2443  Seki J, Ikeda R, Hoshino H.  Dimethyl sulfoxide and related polar compounds enhance infection of human T cells with HIV-1 in vitro  Biochem Biophys Res Commun  1996  227(3):724-9  PubMed ID: 8886001   DOI: 10.1006/bbrc.1996.1576
3853  Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.  Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells  Jpn J Cancer Res  1987  78(10):1112-20  PubMed ID: 2890614  
2609  Hoshino H, Shimizu N, Shimada N, Takita T, Takeuchi T.  Inhibition of infectivity of human immunodeficiency virus by oxetanocin  J Antibiot (Tokyo)  1987  40(7):1077-8  PubMed ID: 3650259   DOI: 10.7164/antibiotics.40.1077
3521  H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura  Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia  Proc Natl Acad Sci U S A  1983  80(19):6061-5  PubMed ID: 6193528   DOI: 10.1073/pnas.80.19.6061
3912  Hoshino H, Shimoyama M, Miwa M, Sugimura T.  Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay  Proc Natl Acad Sci U S A  1983  80(23):7337-41  PubMed ID: 6316359   DOI: 10.1073/pnas.80.23.7337

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