Cell No. : Cell Name
RCB5264 : ATL-3I
update : 2024/01/17
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| Comment | |
| Comment from the depositor | ATL-3I is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-3I cells are positive for HTLV-1 antigens. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
OUE, Atsushi
|
| Originator |
HOSHINO, Hiroo
|
| Year of deposit |
2019
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
71 years
|
| Tissue |
blood
|
| Disease name |
adult T-cell leukemia (ATL)
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Surface antigen (depositor) |
CD4(+), CD3(-), CD8(-)
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_A408
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture medium |
RPMI-1640 + 20% heat-inactivated fetal calf serum
|
RPMI1640 + 20% FBS (heat inactivated)
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| Culture method |
|
|
| Antibiotics |
100units/ml Penicillin G、100μg/ml Streptomycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 2-4 split
|
| SC frequency |
Subculture: 2times/week
|
Subculture : 1-2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
DNA (+)
|
| Virus (EBV) |
|
(-)
|
| STR(human) |
|
OK
|
|
deposit info
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
11
|
8,9
|
9,11
|
9,12
|
16
|
6,9
|
X,Y
|
8,9
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12,13
|
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
2443
Seki J, Ikeda R, Hoshino H.
Dimethyl sulfoxide and related polar compounds enhance infection of human T cells with HIV-1 in vitro
Biochem Biophys Res Commun
1996
227(3):724-9
PubMed ID: 8886001
DOI: 10.1006/bbrc.1996.1576
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3853
Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.
Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells
Jpn J Cancer Res
1987
78(10):1112-20
PubMed ID: 2890614
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2609
Hoshino H, Shimizu N, Shimada N, Takita T, Takeuchi T.
Inhibition of infectivity of human immunodeficiency virus by oxetanocin
J Antibiot (Tokyo)
1987
40(7):1077-8
PubMed ID: 3650259
DOI: 10.7164/antibiotics.40.1077
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3521
H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura
Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia
Proc Natl Acad Sci U S A
1983
80(19):6061-5
PubMed ID: 6193528
DOI: 10.1073/pnas.80.19.6061
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3912
Hoshino H, Shimoyama M, Miwa M, Sugimura T.
Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay
Proc Natl Acad Sci U S A
1983
80(23):7337-41
PubMed ID: 6316359
DOI: 10.1073/pnas.80.23.7337
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