細胞番号 : 細胞名
RCB5263 : ATL-1K
update : 2024/11/11
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細胞特性(Comment:英) | BSL2. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2. |
細胞特性(日) | BSL2. ウイルス産生の有無は不明です。従って、BSL2での取り扱いをお願い致します。 |
細胞特性(寄託者記述:英) | ATL-1K is a cell line established from peripheral blood of a patient with adult T-cell leukemia (ATL) and can grow without IL-2. ATL-1K cells contain one copy of HTLV-1 proviral DNA per cell. The expression of HTLV-1 antigen is not detected. |
細胞特性(寄託者記述:日) | ATL-1Kは、成人T細胞白血病患者の末梢血から樹立されたヒトT細胞白血病ウイルス1型 (HTLV-1: Human T-cell Leukemia Virus Type-1)陽性細胞株。IL-2非依存性に増殖し、1細胞あたり1コピーのHTLV-1 provirusが組み込まれている。ただし、HTLV-1抗原の発現は、検出されない。 |
使用条件(英) | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc Natl Acad Sci U S A 1983 80(19):6061-5) designated by the DEPOSITOR is requested. |
使用条件(日) | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Proc Natl Acad Sci U S A 1983 80(19):6061-5)を引用すること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
Cell line to be handled at BSL 2(C-0070.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
BSL2誓約書C-0069.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
OUE, Atsushi
|
樹立者 |
HOSHINO, Hiroo
|
寄託日 |
2019
|
動物種 |
_human
< Mammals
|
属名 |
Homo
|
種名 |
sapiens
|
人種 |
Japanese
|
性別 |
Male
|
齢 |
82 years
|
採取組織 |
blood
|
病名 |
adult T-cell leukemia (ATL)
|
細胞分類 |
cancer
|
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細胞寿命 |
infinite
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細胞形態 |
lymphocyte-like
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細胞表面抗原(寄託者) |
CD4(+), CD3(-), CD8(-)
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BSL |
BSL2
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Cellosaurus(Expasy) |
CVCL_A406
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
Suspension cells
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Suspension cells
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培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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培地 |
RPMI-1640 + 20% heat-inactivated fetal calf serum
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RPMI1640 + 20% FBS (heat inactivated)
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抗生物質 |
100units/ml Penicillin G、100μg/ml Streptomycin
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Free
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継代方法 |
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dilution
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継代密度 |
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1 : 5-10 split
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継代・培地交換頻度 |
Subculture: 2times/week
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Subculture: 2times/week
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培養最適温度 |
37
℃
|
37
℃
|
二酸化炭素濃度 |
5
%
|
5
%
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凍結培地 |
Medium + 10% DMSO
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Medium + 10% DMSO
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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動物種PCR |
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OK
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ウィルス(HIV) |
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Undetected
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ウィルス(HTLV-1) |
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DNA (+)
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ウィルス(EBV) |
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(-)
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個体識別検査 |
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OK
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寄託時情報
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ロット情報
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D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
10,12
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8
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11
|
11,12
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16,18
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9
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X
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8,11
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12
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文献情報 |
Reference(英) |
14件
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Reference(日) |
0件
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利用者成果(英) |
0件
|
利用者成果(日) |
0件
|
Reference(英) |
10644
Shimizu A, Nakatani Y, Nakamura T, Jinno-Oue A, Ishikawa O, Boeke JD, Takeuchi Y, Hoshino H.
Characterisation of cytoplasmic DNA complementary to non-retroviral RNA viruses in human cells
Sci Rep
2014
4:5074
PubMed ID: 24875540
DOI: 10.1038/srep05074
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6155
Islam S, Hoque SA, Adnan N, Tanaka A, Jinno-Oue A, Hoshino H.
X4-tropic human immunodeficiency virus IIIB utilizes CXCR4 as coreceptor, as distinct from R5X4-tropic viruses
Microbiol Immunol
2013
57(6):437-44
PubMed ID: 23773022
DOI: 10.1111/1348-0421.12051
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2448
Nitta T, Tanaka M, Sun B, Hanai S, Miwa M.
The genetic background as a determinant of human T-cell leukemia virus type 1 proviral load
Biochem Biophys Res Commun
2003
309(1):161-5
PubMed ID: 12943677
DOI: 10.1016/s0006-291x(03)01543-2
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2450
Feng R, Tanaka M, Abe H, Arashi N, Sun B, Uchida K, Miwa M.
Human T-cell leukemia virus type 1 can infect a wide variety of cells in mice
Jpn J Cancer Res
1999
90(1):48-54
PubMed ID: 10076564
DOI: 10.1111/j.1349-7006.1999.tb00664.x
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2455
Hsu DK, Hammes SR, Kuwabara I, Greene WC, Liu FT.
Human T lymphotropic virus-I infection of human T lymphocytes induces expression of the beta-galactoside-binding lectin, galectin-3
Am J Pathol
1996
148(5):1661-70.
PubMed ID: 8623933
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2447
Hori M, Ami Y, Kushida S, Kobayashi M, Uchida K, Abe T, Miwa M.
Intrauterine transmission of human T-cell leukemia virus type I in rats
J Virol
1995
69(2):1302-5
PubMed ID: 7815510
DOI: 10.1128/JVI.69.2.1302-1305.1995
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2707
Kinoshita T, Imamura J, Nagai H, Shimotohno K.
Quantification of gene expression over a wide range by the polymerase chain reaction
Anal Biochem
1992
206(2):231-5
PubMed ID: 1280006
DOI: 10.1016/0003-2697(92)90358-e
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2449
Nyborg JK, Matthews MA, Yucel J, Walls L, Golde WT, Dynan WS, Wachsman W.
Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product
J Biol Chem
1990
265(14):8237-42
PubMed ID: 2186038
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2569
Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T.
Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction
Proc Natl Acad Sci U S A
1989
86(14):5620-4
PubMed ID: 2787512
DOI: 10.1073/pnas.86.14.5620
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2589
Kai C, Okada N, Okada H.
Expression of CR2 (C3d receptor) on the cell membranes of adult T cell leukemia
Jpn J Cancer Res
1988
79(7):805-8
PubMed ID: 2971638
DOI: 10.1111/j.1349-7006.1988.tb00039.x
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3853
Suzuki Y, Hirabayashi Y, Matsumoto N, Kato H, Hidari K, Tsuchiya K, Matsumoto M, Hoshino H, Tozawa H, Miwa M.
Aberrant expression of ganglioside and asialoglycosphingolipid antigens in adult T-cell leukemia cells
Jpn J Cancer Res
1987
78(10):1112-20
PubMed ID: 2890614
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2609
Hoshino H, Shimizu N, Shimada N, Takita T, Takeuchi T.
Inhibition of infectivity of human immunodeficiency virus by oxetanocin
J Antibiot (Tokyo)
1987
40(7):1077-8
PubMed ID: 3650259
DOI: 10.7164/antibiotics.40.1077
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3521
H Hoshino, H Esumi, M Miwa, M Shimoyama, K Minato, K Tobinai, M Hirose, S Watanabe, N Inada, K Kinoshita, S Kamihira, M Ichimaru, T Sugimura
Establishment and characterization of 10 cell lines derived from patients with adult T-cell leukemia
Proc Natl Acad Sci U S A
1983
80(19):6061-5
PubMed ID: 6193528
DOI: 10.1073/pnas.80.19.6061
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3912
Hoshino H, Shimoyama M, Miwa M, Sugimura T.
Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay
Proc Natl Acad Sci U S A
1983
80(23):7337-41
PubMed ID: 6316359
DOI: 10.1073/pnas.80.23.7337
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