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Cell No. : Cell Name
RCB5261 : NCD-2  update : 2024/02/22
Comment
Comment from the depositorHybridoma which produces the rat monoclonal antibody NCD-2 that recognizes chicken N-cadherin.
Terms and conditionsThis resource should be used only for academic research.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor TAKEICHI, Masatoshi
Originator TAKEICHI, Masatoshi
Year of deposit 2019
Animal _mouse x rat < Hybrid
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A6XA
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain rat, Donryu
Fusion_cell_1 gender Female
Fusion_cell_2 name P3-X63-Ag8-U1
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name NCD-2
Subclass IgG2a
Immunogen chicken embryos brain
Antigen_name N-cadherin (chicken)
Antigen_m.w. 135,000
Antigen_character proteins exposed on the cell surface
Antigen_epitope extracellular domain
Antigen_positive nervous tissues cell, connective tissue cell (fibroblast cell)
Antigen_negative epithelial cell
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture medium D-MEM/Ham’F-12 with L-Glutamine and Phenol Red (Cat#048-29785, Wako) + 10% FETAL BOVINE SERUM (Cat# 171012, Cell Culture Bioscience) + 5% Briclone (Archport) DMEM/HamF12 + 10% FBS + 5% Briclone
Culture method
Antibiotics Penicillin , Streptomycin Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER1 Plus (Cat#CB021, ZENOAQ) CELLBANKER 1 Plus
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
12309  K Hatta, M Takeichi  Expression of N-cadherin adhesion molecules associated with early morphogenetic events in chick development  Nature  1986  320(6061):447-9  PubMed ID: 3515198   DOI: 10.1038/320447a0

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