Cell No. : Cell Name
RCB5259 : ECCD-2
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Hybridoma which produces the rat monoclonal antibody ECCD-2 that recognizes E-cadherin. |
| Terms and conditions | This resource should be used only for academic research.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
TAKEICHI, Masatoshi
|
| Originator |
TAKEICHI, Masatoshi
|
| Year of deposit |
2019
|
| Animal |
_mouse x rat
< Hybrid
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A6WX
|
|
hybridoma
|
Fusion_cell_1 name |
spleen
|
| Fusion_cell_1 strain |
rat, Donryu
|
| Fusion_cell_1 gender |
Female
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| Fusion_cell_2 name |
P3-X63-Ag8
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| Fusion_cell_2 strain |
mouse
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|
Fusion method |
HAT selection
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|
Antibody name |
ECCD-2
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| Subclass |
IgG2a
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| Immunogen |
mouse E-cadherin (extracellular domain fragment)
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|
Antigen_name |
E-cadherin (mouse, human, dog, etc)
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| Antigen_m.w. |
124,000
|
| Antigen_character |
proteins exposed on the cell surface
|
| Antigen_epitope |
extracellular domain
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|
Antigen_positive |
epithelial cell
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| Antigen_negative |
fibroblast cell
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
D-MEM/Ham’F-12 with L-Glutamine and Phenol Red (Cat#048-29785, Wako) + 10% FETAL BOVINE SERUM (Cat# 171012, Cell Culture Bioscience) + 5% Briclone (Archport)
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DMEM/HamF12 + 10% FBS + 5% Briclone
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| Antibiotics |
Penicillin , Streptomycin
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Free
|
|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10-20 split
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| SC frequency |
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Subculture : 3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER1 Plus (Cat#CB021, ZENOAQ)
|
CELLBANKER 1 Plus
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Antibody subclass |
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IgG2a(Kappa)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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