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Cell No. : Cell Name
RCB5259 : ECCD-2  update : 2022/09/22
Comment
Comment from the depositorHybridoma which produces the rat monoclonal antibody ECCD-2 that recognizes E-cadherin.
Terms and conditionsThis resource should be used only for academic research.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor TAKEICHI, Masatoshi
Originator TAKEICHI, Masatoshi
Year of deposit 2019
Animal mouse x rat < Hybrid
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A6WX
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain rat, Donryu
Fusion_cell_1 gender Female
Fusion_cell_2 name P3-X63-Ag8
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name ECCD-2
Subclass IgG2a
Immunogen mouse E-cadherin (extracellular domain fragment)
Antigen_name E-cadherin (mouse, human, dog, etc)
Antigen_m.w. 124,000
Antigen_character proteins exposed on the cell surface
Antigen_epitope extracellular domain
Antigen_positive epithelial cell
Antigen_negative fibroblast cell
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives D-MEM/Ham’F-12 with L-Glutamine and Phenol Red (Cat#048-29785, Wako) + 10% FETAL BOVINE SERUM (Cat# 171012, Cell Culture Bioscience) + 5% Briclone (Archport) DMEM/HamF12 + 10% FBS + 5% Briclone
Antibiotics Penicillin , Streptomycin Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER1 Plus (Cat#CB021, ZENOAQ) CELLBANKER 1 Plus
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
12281  A Nagafuchi, Y Shirayoshi, K Okazaki, K Yasuda, M Takeichi  Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA  Nature  1987  329(6137):341-3  PubMed ID: 3498123   DOI: 10.1038/329341a0
12307  Y Shirayoshi, A Nose, K Iwasaki, M Takeichi  N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin  Cell Struct Funct  1986  11(3):245-52  PubMed ID: 3768962   DOI: 10.1247/csf.11.245

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