Cell No. : Cell Name
RCB5257 : AS-E2
update : 2024/04/01
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| Comment | |
| Comment from the depositor | An erythropoietin-dependent human leukemia cell line AS-E2 has been established from bone marrow of pure erythroid leukemia (PEL) patient. AS-E2 cells are strictly dependent on erythropoietin (EPO) for their growth and survival. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Leukemia 1997 11(11):1941-9) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
MIYAZAKI, Yasushi
|
| Originator |
MIYAZAKI, Yasushi
|
| Year of deposit |
2019
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
62 years
|
| Tissue |
bone marrow
|
| Disease name |
pure erythroid leukemia（PEL)
|
| Classification |
cancer
|
| Year of origin |
1993
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_6769
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture medium |
IMDM with 20% heat inactivated FBS + EPO 2U/ml
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IMDM (4mM L-Glutamine) + 20% FBS (heat inactivated) + 2U/ml EPO
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| Culture method |
|
|
| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
|
Passage cell No |
1ｘ10 5−1.5ｘ10 5 /ml
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|
| Passage ratio |
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1 : 2-5 split
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| SC frequency |
Subculture: 1:5-2:5 every 5days
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
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| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Virus (EBV) |
|
Undetected
|
| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
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| Reference |
|
10956
Yoriko Inoue, Hideki Tsushima, Koji Ando, Yasushi Sawayama, Mari Sakai, Reishi Yamasaki, Emi Matsuo, Chizuko Tsutsumi, Yoshitaka Imaizumi, Masako Iwanaga, Daisuke Imanishi, Jun Taguchi, Yasushi Miyazaki, Masao Tomonaga
Chemokine expression in human erythroid leukemia cell line AS-E2: macrophage inflammatory protein-3alpha/CCL20 is induced by inflammatory cytokines
Exp Hematol
2006
34(1):19-26
PubMed ID: 16413387
DOI: 10.1016/j.exphem.2005.09.012
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10419
H Tsushima, Y Urata, Y Miyazaki, K Fuchigami, K Kuriyama, T Kondo, M Tomonaga
Human erythropoietin receptor increases GATA-2 and Bcl-xL by a protein kinase C-dependent pathway in human erythropoietin-dependent cell line AS-E2
Cell Growth Differ
1997
8(12):1317-28
PubMed ID: 9419420
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9999
Y Miyazaki, K Kuriyama, M Higuchi, H Tsushima, H Sohda, N Imai, M Saito, T Kondo, M Tomonaga
Establishment and characterization of a new erythropoietin-dependent acute myeloid leukemia cell line, AS-E2
Leukemia
1997
11(11):1941-9
PubMed ID: 9369430
DOI: 10.1038/sj.leu.2400838
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