Cell No. : Cell Name
RCB5125 : Rab25-KO MDCK-II
update : 2024/11/11
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| Comment | |
| Comment from the depositor | MDCK-II cells (Rab-knockout clone ). J Cell Biol. 2019; 218(6): 2035-2050, Tables S1-S3 (ZIP) |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Cell Biol 2019 218(6):2035-2050) designated by the DEPOSITOR is requested.
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| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
FUKUDA, Mitsunori
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| Originator |
HOMMA, Yuta
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| Year of deposit |
2019
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| Original cell |
MDCK-II (RCB5148)
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| Animal |
_dog
< Mammals
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| Genus |
Canis
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| Species |
lupus familiaris
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| Gender |
Female
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| Tissue |
kidney
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| Classification |
transformed
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| Recombinant |
recombinant
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| Vector |
pSpCas9(BB)-2A-Puro vector
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| Recombinant virus |
No
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_A6VR
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM (044-29765, FUJIFILM Wako Pure Chemical) + 10% FBS
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DMEM (high glucose, without Sodium pyruvate) + 10% FBS
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| Antibiotics |
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Free
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Passage method |
0.05% trypsin-EDTA (25200-056, GIBCO)
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage cell No |
1×10 5cells/ 6 cm dish
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1x10 5 cells / 60mm dish
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| SC frequency |
2-3 times/week
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Subculture : 2-3 times/week
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| Temperature |
37
℃
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37
℃
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| CO2 concentration |
5
%
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5
%
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| Freeze medium |
CELLBANKER 1plus (CB021, NIPPON ZENYAKU KOGYO CO., LTD.)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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| Animal PCR |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
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| User's Publication |
0
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| Reference |
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17829
Trofimenko E, Homma Y, Fukuda M, Widmann C.
The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
Cell Rep
2021
37(5):109945.
PubMed ID: 34731620
DOI: 10.1016/j.celrep.2021.109945
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10577
Yuta Homma, Shu Hiragi, Mitsunori Fukuda
Rab Family of Small GTPases: An Updated View on Their Regulation and Functions
FEBS J
2020
288(1):36-55
PubMed ID: 32542850
DOI: 10.1111/febs.15453
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6046
Homma Y, Kinoshita R, Kuchitsu Y, Wawro PS, Marubashi S, Oguchi ME, Ishida M, Fujita N, Fukuda M.
Comprehensive knockout analysis of the Rab family GTPases in epithelial cells.
J Cell Biol
2019
218(6):2035-2050
PubMed ID: 31072826
DOI: 10.1083/jcb.201810134
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