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Cell No. : Cell Name
RCB5116 : Rab15-KO MDCK-II  update : 2021/05/21
Comment
Comment from the depositorMDCK-II cells (Rab-knockout clone ). J Cell Biol. 2019; 218(6): 2035-2050, Tables S1-S3 (ZIP)
Terms and conditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Cell Biol 2019 218(6):2035-2050) designated by the DEPOSITOR is requested.
RemarksCRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. : MTA (C-0065) For use for not-for-profit academic purpose.
Order Form Order Form(C-0005.pdf)   MTA(C-0065.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUKUDA, Mitsunori
Originator HOMMA, Yuta
Year of deposit 2019
Original cell MDCK-II (RCB5148)
Animal _dog < Mammals
Genus Canis
Species lupus familiaris
Gender Female
Tissue kidney
Classification transformed
Recombinant recombinant
Vector pSpCas9(BB)-2A-Puro vector
Recombinant virus No
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A6VD
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives DMEM (044-29765, FUJIFILM Wako Pure Chemical) + 10% FBS DMEM (high glucose, without Sodium pyruvate) + 10% FBS
Antibiotics Free
Passage method 0.05% tripsin-EDTA (25200-056, GIBCO) 0.25% Trypsin + 0.02% EDTA
Culture information Passage cell No 1×10^5 cells / 6 cm dish 1x10^(5) cells / 60mm dish
SC frequency 2-3 times/week Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1plus (CB021, NIPPON ZENYAKU KOGYO CO., LTD.) CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
Reference information Reference 2
User's Publication 0


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Reference
10577  Yuta Homma, Shu Hiragi, Mitsunori Fukuda  Rab Family of Small GTPases: An Updated View on Their Regulation and Functions  FEBS J  2020  Jun 15  PubMed ID: 32542850
6046  Homma Y, Kinoshita R, Kuchitsu Y, Wawro PS, Marubashi S, Oguchi ME, Ishida M, Fujita N, Fukuda M.  Comprehensive knockout analysis of the Rab family GTPases in epithelial cells.  J Cell Biol  2019  218(6):2035-2050  PubMed ID: 31072826

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