Cell No. : Cell Name
RCB5095 : KIF13-DKO HeLaM
update : 2024/11/11
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| Comment | |
| Comment from the depositor | KIF13A and KIF13B double knockout (DKO) HeLaM cells. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Cell Sci 2019 132(5). pii: jcs226977) designated by the DEPOSITOR is requested.
|
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
FUKUDA, Mitsunori
|
| Originator |
ETOH, Kan
|
| Year of deposit |
2019
|
| Original cell |
HeLaM
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Vector |
pSpCas9(BB)-2A-Puro vector
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A7AB
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
|
| Culture medium |
DMEM (044-29765, FUJIFILM Wako Pure Chemical) + 10% FBS
|
DMEM (high glucose, without Sodium pyruvate) + 10% FBS
|
| Antibiotics |
100 U/ml penicillin G, and 100 μg/ml streptomycin
|
Free
|
|
Passage method |
0.05% trypsin-EDTA (25200-056, GIBCO)
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
|
Passage cell No |
2×10 5cells/ 6 cm dish
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(6-8x10 5 cells / 100mm dish) or (1 : 10-20)
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| SC frequency |
2-3 times/week
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Subculture : 2-3 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER 1plus (CB021, NIPPON ZENYAKU KOGYO CO., LTD.)
|
CELLBANKER 1
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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