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Cell No. : Cell Name
RCB5095 : KIF13-DKO HeLaM  update : 2024/01/17
Comment
Comment from the depositorKIF13A and KIF13B double knockout (DKO) HeLaM cells.
Terms and conditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Cell Sci 2019 132(5). pii: jcs226977) designated by the DEPOSITOR is requested.
RemarksCRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form Order Form(C-0005.pdf)   MTA(C-0065.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUKUDA, Mitsunori
Originator ETOH, Kan
Year of deposit 2019
Original cell HeLaM
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Vector pSpCas9(BB)-2A-Puro vector
Recombinant virus No
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7AB
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM (044-29765, FUJIFILM Wako Pure Chemical) + 10% FBS DMEM (high glucose, without Sodium pyruvate) + 10% FBS
Antibiotics 100 U/ml penicillin G, and 100 μg/ml streptomycin Free
Passage method 0.05% trypsin-EDTA (25200-056, GIBCO) 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage cell No 2×10 5cells/ 6 cm dish (6-8x10 5 cells / 100mm dish) or (1 : 10-20)
SC frequency 2-3 times/week Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1plus (CB021, NIPPON ZENYAKU KOGYO CO., LTD.) CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
6047  Etoh K, Fukuda M.  Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors.  J Cell Sci  2019  132(5). pii: jcs226977  PubMed ID: 30700496   DOI: 10.1242/jcs.226977

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