Cell No. : Cell Name
RCB5056 : DP028
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Dental pulp cells are derived from human dental pulp. They are characterized with fibroblastic morphology, and contamination of hematopoetic cells is minimal. |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Dent Res. 2008;87(7):676-681) designated by the DEPOSITOR is required. 2) Attach Appendix to MTA (C-0007 or C-0007p) . |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
TEZUKA, Ken-ichi
|
| Originator |
TEZUKA, Ken-ichi
|
| Year of deposit |
2019
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
14 years
|
| Tissue |
Dental pulp
|
| Classification |
normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
finite
|
| Crisis PDL |
60-66
PDL
|
| Morphology |
fibroblast-like
|
| Differentiation |
Differentiation towards odontoblasts induced by confluency.
|
| Biochemical |
Nestin (+)
|
| Immunological |
HLA Class I (+)
|
| Surface antigen (depositor) |
CD73, CD90, CD105
|
| Contact inhibition |
Yes
|
| Tumorigenesis |
No
|
| Cellosaurus(Expasy) |
CVCL_RM96
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
LONZA MSCGM PT-3001
|
MSCGM (LONZA PT-3001)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Designated culture dish |
Tissue Culture Dish (Falcon)
|
|
Culture information
|
Passage cell No |
5000-6000 cells/cm 2
|
5000-6000 cells/cm 2
|
| SC frequency |
|
Subculture : once/3-4 days
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER 2
|
CELLBANKER 2
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
(-)
|
|
| Virus (HTLV-1) |
(-)
|
|
| Chromosome mode |
46,XY
|
|
| Saturation density |
2-4 x 10 4 cells/cm 2
|
|
| Doubling time |
38
hr
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
7524
N Tamaoki, K Takahashi, T Tanaka, T Ichisaka, H Aoki, T Takeda-Kawaguchi, K Iida, T Kunisada, T Shibata, S Yamanaka, K Tezuka
Dental pulp cells for induced pluripotent stem cell banking
J Dent Res
2010
89(8):773-8
PubMed ID: 20554890
DOI: 10.1177/0022034510366846
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|
7544
T Takeda, Y Tezuka, M Horiuchi, K Hosono, K Iida, D Hatakeyama, S Miyaki, T Kunisada, T Shibata, K Tezuka
Characterization of dental pulp stem cells of human tooth germs
J Dent Res
2008
87(7):676-81
PubMed ID: 18573990
DOI: 10.1177/154405910808700716
|
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