Cell No. : Cell Name
RCB5036 : CMG14-12
update : 2025/05/19
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| Comment | |
| Comment from the depositor | Mouse M-CSF producing Ltk- cell line, CMG14-12. The mouse M-CSF expression vector was transfected into mouse Ltk- cells, M-CSF high producing cel l line was selected and named ''CMG14-12''. CMG14-12: M-CSF producing cell line |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Bone Miner Res. 2000;15(8):1477-88) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
TAKESHITA, Sunao
|
| Originator |
TAKESHITA, Sunao
|
| Year of deposit |
2018
|
| Original cell |
L-M(TK-)
|
| Cloning (depositor) |
Yes
|
| _Method (depositor) |
limiting dilution
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/An
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
mouse M-CSF cDNA
|
|
1.9K
base
|
| Vector |
pCXN2
|
| Selection |
G418 (400μg/ml)
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_A9A2
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM(Sigma D5796), 10%FBS CMG14-12: M-CSF producing cell line
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DMEM (high glucose, without Sodium pyruvate) + FBS (heat inactivated) + 400μg/ml G418 (Without G418 when thawing. Add it next day.)
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| Antibiotics |
Pen/Sm
|
Free
|
|
Passage method |
|
0.05% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage cell No |
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1x10 6cells/100mm dish
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
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| SSLP(mouse) |
|
OK
|
| Doubling time |
20
hr
|
|
| Reference information |
Reference |
1
|
| User's Publication |
0
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