Cell No. : Cell Name
RCB5029 : NIH3T3 Flp Arl13bCerulean-Fucci2a
update : 2024/07/31
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| Comment | Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | NIH/3T3 is a fibroblast cell line that was isolated from a mouse NIH/Swiss embryo. These cells were generated by a stable integration of pCDNA5-CAG-Arl13bCerulean-Fucci2a using the Flp-in system they were not cloned but are isogenic. |
| Terms and conditions | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Dev Cell 2018 47(4):509-523.e5) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
University of Edinburgh (UK)
|
| Depositor |
Ian, Jackson
|
| Originator |
Ian, Jackson
|
| Year of deposit |
2018
|
| Original cell |
NIH/3T3
|
| Cloning (depositor) |
No
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NIH/Swiss
|
| Genus |
Mus
|
| Species |
musculus
|
| Gender |
Unknown
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pCDNA5-CAG-Arl13bFucci2a
|
| Selection |
100 μg/ml Hygromycin (Gibco)
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
Dulbecco’s modified Eagle’s medium (DMEM) containing ; 10% fetal calf serum (FCS), 1% Penicillin/Streptomycin, 25 mM D-Glucose, 4 mM L-glutamine, 1 mM sodium pyruvate
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage cell No |
|
3 x 10 5 cells/60 mm dish
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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