Cell No. : Cell Name
RCB5010 : D4-ChAT
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | Hybridoma cell line producing anti-chicken ChAT antibody (mouse IgG). |
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Dev Growth Differ 2017 59(9):677-687) designated by the DEPOSITOR is requested. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
TAKAHASHI, Yoshiko
|
| Originator |
TAKAHASHI, Yoshiko
|
| Year of deposit |
2018
|
| Another name |
D4
|
| Cloning (depositor) |
No
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
| Recombinant |
non-recombinant
|
| Year of origin |
2015
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Contact inhibition |
No
|
| Tumorigenesis |
No
|
|
hybridoma
|
Fusion_cell_1 name |
P3U1
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_1 gender |
Unknown
|
| Fusion_cell_2 name |
spleen
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
| Fusion_cell_2 gender |
Unknown
|
|
Fusion method |
HAT
|
|
Antibody name |
D4
|
| Subclass |
IgG
|
|
Antigen_name |
ChAT
|
| Antigen_m.w. |
46kDa, 47kDa, 70kDa
|
| Antigen_character |
peptide SYLPGHTKDTLRSD
|
| Antigen_epitope |
N-terminus
|
|
Antigen_positive |
nerve, heart
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI1640 + 15% FBS
|
RPMI1640 + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
twice/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Antibody subclass |
|
IgG2a(Kappa)
|
| Saturation density |
5x10 6 cells/ml
|
|
| Doubling time |
12
hr
|
|
| Plating efficiency |
50
%
|
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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