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Cell No. : Cell Name
RCB5008 : MCC148c update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | Human cell line derived from poor differentiated lung squamous carcinoma. Transplantable to NOG mice. | |||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Lab Invest 2019 99(9):1349-1362) designated by the DEPOSITOR is requested. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | TAMAI, Keiici | ||
| Originator | MOCHIZUKI, Mai | |||
| Year of deposit | 2018 | |||
| Cloning (depositor) | No | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Male | |||
| Age at sampling | 84 years | |||
| Tissue | lung | |||
| Tumor | T1bNOMO, poor differentiated lung SCC | |||
| Metastatic ability | Unknown | |||
| Classification | cancer | |||
| Recombinant | non-recombinant | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Tumorigenesis | Yes | |||
| Cellosaurus(Expasy) | CVCL_A7AR | |||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | DMEM (high glucose) 10%FBS, 2.5μM Y-27632, 0.4μg/ml Hydrocortisone | DMEM (high glucose) + 10%FBS + 2.5μM Y-27632 + 0.4μg/ml Hydrocortisone | ||
| Antibiotics | Pn/St | Free | Passage method | 0.05% trypsin | 0.25% Trypsin | Culture information | Passage cell No | 3-4x10 6/10cm dish |
| Passage ratio | 1 : 2-3 | |||
| SC frequency | Subculture : twice/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Cell banker 1 | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Virus (HIV) | (-) | |||
| Virus (HBV) | (-) | |||
| Virus (HCV) | (-) | |||
| Virus | Sendai virus (-), LCM virus (-) | |||
| Doubling time | 48 hr | |||
| STR(human) | OK | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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