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Cell No. : Cell Name
RCB5005 : FKH-1  update : 2024/11/11
CommentAcute myeloid leukemia with dek/can chimera mRNA expression. Responding to G-CSF and differentiate.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.HAMAGUCHI Hiroyuki (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor HAMAGUCHI, Hiroyuki
Originator HAMAGUCHI, Hiroyuki
Year of deposit 1998
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 60 years
Tissue peripheral blood
Disease name AML
Classification cancer
Year of origin 1993
Lifespan infinite
Morphology other (blast-like)
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS (heat inactivated) + 0.1mM NEAA + 5-10ng/ml human G-CSF RPMI1640 + 10% FBS (heat inactivated) + 0.1mM NEAA + 10ng/ml human GM-CSF
Antibiotics Free Free
Passage method dilution dilution
Culture information Passage ratio 1 : 2 split 1 : 2-4 split
SC frequency Subculture : once/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Virus (EBV) (-)
Chromosome mode 45, XY, t(6;9)(p23;q34), -7
Surface antigen CD13, CD33
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2346  Hamaguchi H, Nagata K, Yamamoto K, Fujikawa I, Kobayashi M, Eguchi M  Establishment of a novel human myeloid leukaemia cell line (FKH-1) with t(6;9)(p23;q34) and the expression of dek-can chimaeric transcript.  Br J Haematol  1998  102:1249-56  PubMed ID: 9753053   DOI: 10.1046/j.1365-2141.1998.00900.x

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