Cell No. : Cell Name
RCB5004 : CHL-YN
update : 2024/11/11
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| Comment | Application consideration |
| Comment from the depositor | Novel cells derived from female Chinese hamster lung. Cells were immortalized by spontaneous transformation. Cells were adapted in animal component-free chemically defined media and subjected to biosafety testing (BioReliance), in which safety was confirmed by an in vitro assay to detect viral contaminants using 3 detector cell lines, a mycoplasma detection test by real-time PCR, and sterility testing by direct inoculation. |
| Terms and conditions | Applies only for research purpose. If the purpose of these cells and any transformations involving these cells are for business, approval from Manufacturing Technology Association of Biologics, the providers of these cells, must be gotten beforehand. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
YAMANO, Noriko & IWAKURA, Masahiro & OMASA, Takeshi
|
| Originator |
YAMANO, Noriko
|
| Year of deposit |
2018
|
| Another name |
Chinese hamster lung-YN
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| Cloning (depositor) |
No
|
| Animal |
_hamster
< Mammals
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| Strain name |
Chinese
|
| Genus |
Cricetulus
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| Species |
griseus
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| Gender |
Female
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| Tissue |
lung
|
| Classification |
normal
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| Recombinant |
non-recombinant
|
| Year of origin |
2014
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|
Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_YS93
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
EX-CELL CD CHO FUSION : SIGMA-ALDRICH (14365C) + 6mM L-Glutamine
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EX-CELL CD CHO Fusion + 6mM L-Glutamine
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| Antibiotics |
Free
|
Free
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|
Passage method |
dilution
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dilution
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| Designated culture dish |
Erlenmeyer flask / shaking culture（φ25 mm, 140 rpm). Erlenmeyer flask / shaking culture (φ50 mm, 80-90 rpm) are also acceptable.
|
erlenmeyer flask / shaking culture (80 rpm)
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Culture information
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Passage cell No |
5×10 4 cells/mL (next passaging 4 days later, 1×10 5 cells/mL (next passaging 3 days later)
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5×10 4 cells/mL (next passaging 4 days later), 1×10 5 cells/mL (next passaging 3 days later)
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
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| Freeze medium |
Cell Reservoir One (with DMSO)
|
Cell Reservoir One (with DMSO)
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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|
| Animal PCR |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
1
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