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Cell No. : Cell Name
RCB5003 : IWT-1  update : 2024/07/09
Comment
Comment from the depositorThis cell line was established from gastric adenocarcinoma cells in peritonitis carcinomatosa ascites.
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor NISHIZUKA, Satoshi
Originator NISHIZUKA, Satoshi
Year of deposit 2018
Another name IWT1
Cloning (depositor) No
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 58 years
Tissue peritonitis carcinomatosa ascites
Primary focus gastric cancer
Disease name gastric cancer
Tumor gastric adenocarcinoma, Stage IV
Metastatic ability Unknown
Classification cancer
Recombinant non-recombinant
Year of origin 2010
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A6JG
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent/Suspension cells
Culture medium RPMI-1640 + 10%FBS RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin 0.25% Trypsin
Culture information Passage cell No 1.0-5.0 x 10 5 cells/25 cm 2
Passage ratio 1:4 1 : 4 split
SC frequency twice/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HBV) patient : (-)
Virus (HCV) patient : (-)
Virus (EBV) (-)
Saturation density 2.0-3.0 x 10 6 cells/25 cm 2
Doubling time 22.7 hr
Others GI50 of 5-FU : 15μM. TP53 codon72 : Arg/Arg
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
3286  Endo F, Nishizuka SS, Kume K, Ishida K, Katagiri H, Ishida K, Sato K, Iwaya T, Koeda K, Wakabayashi G.  A compensatory role of NF-κB to p53 in response to 5-FU-based chemotherapy for gastric cancer cell lines  PLoS One  2014  9(2):e90155  PubMed ID: 24587255   DOI: 10.1371/journal.pone.0090155

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