Cell No. : Cell Name
RCB5003 : IWT-1
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | This cell line was established from gastric adenocarcinoma cells in peritonitis carcinomatosa ascites. |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
NISHIZUKA, Satoshi
|
| Originator |
NISHIZUKA, Satoshi
|
| Year of deposit |
2018
|
| Another name |
IWT1
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
58 years
|
| Tissue |
peritonitis carcinomatosa ascites
|
| Primary focus |
gastric cancer
|
| Disease name |
gastric cancer
|
| Tumor |
gastric adenocarcinoma, Stage IV
|
| Metastatic ability |
Unknown
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
| Year of origin |
2010
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A6JG
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent/Suspension cells
|
| Culture medium |
RPMI-1640 + 10%FBS
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
0.25% trypsin
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
1.0-5.0 x 10 5 cells/25 cm 2
|
|
| Passage ratio |
1:4
|
1 : 4 split
|
| SC frequency |
twice/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HBV) |
patient : (-)
|
|
| Virus (HCV) |
patient : (-)
|
|
| Virus (EBV) |
|
(-)
|
| Saturation density |
2.0-3.0 x 10 6 cells/25 cm 2
|
|
| Doubling time |
22.7
hr
|
|
| Others |
GI50 of 5-FU : 15μM. TP53 codon72 : Arg/Arg
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
Back
Top
CELL BANK Website