Cell No. : Cell Name
RCB4965 : FBD-102b
update : 2024/12/09
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| Comment | |
| Comment from the depositor | Clonal oligodendroglial progenitor cell line established from brain of fetal p53-/- mice. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Cell Struct Funct 1998;23(2):101-7, Neurosci Res 2006;56(2):213-9) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
TOMOOKA, Yasuhiro
|
| Originator |
TOMOOKA, Yasuhiro
|
| Year of deposit |
2018
|
| Animal |
_mouse
< Mammals
|
| Strain name |
Mus
|
| Genus |
musculus
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| Species |
p53-/- mouse (hybrid between C57BL/6 and CBA)
|
| Age at sampling |
13th day of pregnancy
|
| Tissue |
brain
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
Neomycin resistance gene
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Differentiation |
Process elongation and cell proliferation of astrocytes are induced by co-culture with FBD-102b cells.
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| Biochemical |
FBD-102b cells respond to semaphorin.
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| Cellosaurus(Expasy) |
CVCL_C6DV
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM/F12, 10% fetal bovine serum (biosera), growth stimulation : + 10 μg/ml insulin + 10 μg/ml transferrin + 10 -8M sodium selenite + 1 μM forskolin
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DMEM/HamF12 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
Trypsin EDTA
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage ratio |
1 : 10
|
1 : 5-10
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
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| Reference |
|
21022
Tomohiro Torii, Remina Shirai, Risa Kiminami, Satoshi Nishino, Takanari Sato, Sui Sawaguchi, Nana Fukushima, Yoichi Seki, Yuki Miyamoto , Junji Yamauchi
Hypomyelinating Leukodystrophy 10 (HLD10)-Associated Mutations of PYCR2 Form Large Size Mitochondria, Inhibiting Oligodendroglial Cell Morphological Differentiation
Neurol Int
2022
14(4):1062-1080
PubMed ID: 36548190
DOI: 10.3390/neurolint14040085
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|
1915
Okada A, Tomooka Y.
A role of Sema6A expressed in oligodendrocyte precursor cells
Neurosci Lett
2013
539:48-53
PubMed ID: 23376059
DOI: 10.1016/j.neulet.2013.01.026
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|
1914
Horiuchi M, Tomooka Y.
An oligodendroglial progenitor cell line FBD-102b possibly secretes a radial glia-inducing factor
Neurosci Res
2006
56(2):213-9
PubMed ID: 16884801
DOI: 10.1016/j.neures.2006.06.007
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|
1911
Tomooka Y, Aizawa S.
Cell lines established from fetal brains of p53-deficient mice
Cell Struct Funct
1998
23(2):101-7
PubMed ID: 9669038
DOI: 10.1247/csf.23.101
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