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Cell No. : Cell Name
RCB4942 : BHK/T7-9  update : 2024/02/13
Comment
Comment from the depositorA hamster kidney-derived BHK cell clone stably expressing T7 RNA polymerase.
Terms and conditions1) RECIPIENT shall be a non-profit research organization, or a university or other institution of higher education, or a government agency conducting research. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Microbiol Immunol. 2003 47(8):613-617) designated by the DEPOSITOR is required. 3) RECIPIENT shall use the BI0LOGICAL RESOURCE solely for internal research which means cultivation, storage and accessibility test. 4) RECIPIENT shall not undertake the Research jointly with any third parties or entrust the Research to any third parties without the prior written consent of DEPOSITOR. 5) RECIPIENT shall neither use the BIOLOGICAL RESOURCE for any purpose other than the Research nor provide the Material to any third parties without the prior written consent of DEPOSITOR. 6) The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 7) When publishing the Research and the Research Results, RECIPIENT shall notify DEPOSITOR in writing in advance of such publication. the timing, and method, etc., and shall consult with DEPOSITOR. 8) When Publishing as provided for in the preceding paragraph, RECIPIENT shall, in accordance with a request from DEPOSITOR/DEVELOPER, acknowledge that DEVELOPER ( Dr. Naoto Ito and Dr. Makoto Sugiyama) is the source of the BIOLOGICAL RESOURCE.
Remarks
approver's address
×
English
Address
Gifu University, 1-1 Yanagido, Gifu City 501-1193, JAPAN
Dr.Ito Naoto
Fax. +81-58-293-2949
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ITO, Naoto
Originator ITO, Naoto
Year of deposit 2018
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification other
Recombinant recombinant
Exogene T7 RNA polymerase gene
2.9K base
Vector pCAGGS/MCS
Selection 600 μg/ml hygromycin (every 10 passages)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A8V7
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium Eagle’s MEM with 5% Fetal Calf Serum and 10% Tryptose Phosphate Broth (BD, Difco 260300) MEM + 5% FBS + 10% Tryptose Phosphate Broth ( Media preparation )
Antibiotics 100 U/ml penicillin and 100 U/ml streptomycin Free
Passage method Trypsin-EDTA 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Designated culture dish 100 mm dish
Culture information Passage ratio 1:5 – 1:10 1 : 8 split
SC frequency Subculture : once/4 days Subculture : once/4 days
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
4556  Ito N, Takayama-Ito M, Yamada K, Hosokawa J, Sugiyama M, Minamoto N.  Improved recovery of rabies virus from cloned cDNA using a vaccinia virus-free reverse genetics system.  Microbiol Immunol.  2003  47(8):613-617  PubMed ID: 14524622   DOI: 10.1111/j.1348-0421.2003.tb03424.x

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User's Publication
19812  Watanabe Y, Sakuma C, Yaginuma H.  Tangentially migrating cells differentiate into stratum griseum central neurons to form tectofugal visual pathway in the developing chick optic tectum  Dev Dyn  2023  252(8):1096-1112  PubMed ID: 36734001   DOI: 10.1002/dvdy.572
21144  Itakura Y, Tabata K, Morimoto K, Ito N, Chambaro HM, Eguchi R, Otsuguro KI, Hall WW, Orba Y, Sawa H, Sasaki M.  Glu333 in rabies virus glycoprotein is involved in virus attenuation through astrocyte infection and interferon responses.  iScience  2022  25(4):104122  PubMed ID: 35402872   DOI: 10.1016/j.isci.2022.104122
16286  Yun SM, Lee TY, Lim HY, Ryou J, Lee JY, Kim YE.  Development and Characterization of a Reverse Genetics System for a Human-Derived Severe Fever With Thrombocytopenia Syndrome Virus Isolate From South Korea  Front Microbiol  2021  12:772802  PubMed ID: 34867909   DOI: 10.3389/fmicb.2021.772802



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