Cell No. : Cell Name
RCB4940 : bTS5
update : 2024/11/11
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| Comment | TS culture protocol, TS culture protocol_V2 |
| Comment from the depositor | Human trophoblast stem cells were derived from blastocysts or placental cytotrophoblast cells. Human trophoblast stem cells have the potential to differentiate into extravillous cytotrophoblast and syncytiotrophoblast cells. |
| Terms and conditions | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell. 2018 ;22(1):50-63) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ARIMA, Takahiro
|
| Originator |
ARIMA, Takahiro
|
| Year of deposit |
2018
|
| Another name |
TS^(blast)#2
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
blastocyst
|
| Classification |
normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
finite
|
| Crisis PDL |
150
PDL
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A6JH
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
Please check [Comment].
|
Please check [Comment].
|
| Antibiotics |
|
Free
|
|
Passage method |
Please check [Comment].
|
Please check [Comment].
|
Culture information
|
Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER 1
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
(-)
|
|
| Virus (HTLV-1) |
(-)
|
|
| Virus (HBV) |
(-)
|
|
| Virus (HCV) |
(-)
|
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
3
|
| Reference |
|
4606
Takahashi S, Okae H, Kobayashi N, Kitamura A, Kumada K, Yaegashi N, Arima T.
Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells.
Proc Natl Acad Sci U S A
2019
116(52):26606-26613
PubMed ID: 31792181
DOI: 10.1073/pnas.1916019116
|
|
4566
Okae H, Toh H, Sato T, Hiura H, Takahashi S, Shirane K, Kabayama Y, Suyama M, Sasaki H, Arima T.
Derivation of Human Trophoblast Stem Cells.
Cell Stem Cell.
2018
22:50-63
PubMed ID: 29249463
DOI: 10.1016/j.stem.2017.11.004
|
| User's Publication |
|
19851
Baird L, Cannon P, Kandel M, Nguyen TV, Nguyen A, Wong G, Murphy C, Brownfoot FC, Kadife E, Hannan NJ, Tong S, Bartho LA, Kaitu'u-Lino TJ.
Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia
Reprod Biol Endocrinol
2023
21(1):65
PubMed ID: 37464405
DOI: 10.1186/s12958-023-01116-3
|
|
19852
Nguyen A, Cannon P, Kandel M, Nguyen TV, Baird L, Wong G, Hannan NJ, Tong S, Bartho L, Kaitu'u-Lino TJ.
Cell surface associated protein mucin 15 (MUC15) is elevated in preeclampsia
Placenta
2023
140:39-46
PubMed ID: 37531748
DOI: 10.1016/j.placenta.2023.07.013
|
|
19854
Bartho LA, Kandel M, Walker SP, Cluver CA, Hastie R, Bergman L, Pritchard N, Cannon P, Nguyen TV, Wong GP, MacDonald TM, Keenan E, Hannan NJ, Tong S, Kaitu'u-Lino TJ.
Circulating Chemerin Is Elevated in Women With Preeclampsia
Endocrinology
2023
164(5):bqad041
PubMed ID: 36882076
DOI: 10.1210/endocr/bqad041
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