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Cell No. : Cell Name
RCB4937 : CT29  update : 2024/07/09
CommentTS culture protocol, TS culture protocol_V2
Comment from the depositorHuman trophoblast stem cells were derived from blastocysts or placental cytotrophoblast cells. Human trophoblast stem cells have the potential to differentiate into extravillous cytotrophoblast and syncytiotrophoblast cells.
Terms and conditions1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell. 2018 ;22(1):50-63) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ARIMA, Takahiro
Originator ARIMA, Takahiro
Year of deposit 2018
Another name TS^(CT)#2
Cloning (depositor) No
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue placenta (Cytotrophoblast cells)
Classification normal
Recombinant non-recombinant
Lifespan finite
Crisis PDL 150 PDL
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7BA
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium Please check [Comment]. Please check [Comment].
Antibiotics Free
Passage method Please check [Comment]. Please check [Comment].
Culture information Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Virus (HBV) (-)
Virus (HCV) (-)
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 6

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4606  Takahashi S, Okae H, Kobayashi N, Kitamura A, Kumada K, Yaegashi N, Arima T.  Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells.  Proc Natl Acad Sci U S A  2019  116(52):26606-26613  PubMed ID: 31792181   DOI: 10.1073/pnas.1916019116
4566  Okae H, Toh H, Sato T, Hiura H, Takahashi S, Shirane K, Kabayama Y, Suyama M, Sasaki H, Arima T.  Derivation of Human Trophoblast Stem Cells.  Cell Stem Cell.  2018  22:50-63  PubMed ID: 29249463   DOI: 10.1016/j.stem.2017.11.004

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User's Publication
21593  Shannon MJ, McNeill GL, Koksal B, Baltayeva J, Wächter J, Castellana B, Peñaherrera MS, Robinson WP, Leung PCK, Beristain AG.  Single-cell assessment of primary and stem cell-derived human trophoblast organoids as placenta-modeling platforms.  Dev Cell  2024    PubMed ID: 38359834   DOI: 10.1016/j.devcel.2024.01.023
19851  Baird L, Cannon P, Kandel M, Nguyen TV, Nguyen A, Wong G, Murphy C, Brownfoot FC, Kadife E, Hannan NJ, Tong S, Bartho LA, Kaitu'u-Lino TJ.  Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia  Reprod Biol Endocrinol  2023  21(1):65  PubMed ID: 37464405   DOI: 10.1186/s12958-023-01116-3
19852  Nguyen A, Cannon P, Kandel M, Nguyen TV, Baird L, Wong G, Hannan NJ, Tong S, Bartho L, Kaitu'u-Lino TJ.  Cell surface associated protein mucin 15 (MUC15) is elevated in preeclampsia  Placenta  2023  140:39-46  PubMed ID: 37531748   DOI: 10.1016/j.placenta.2023.07.013
19854  Bartho LA, Kandel M, Walker SP, Cluver CA, Hastie R, Bergman L, Pritchard N, Cannon P, Nguyen TV, Wong GP, MacDonald TM, Keenan E, Hannan NJ, Tong S, Kaitu'u-Lino TJ.  Circulating Chemerin Is Elevated in Women With Preeclampsia  Endocrinology  2023  164(5):bqad041  PubMed ID: 36882076   DOI: 10.1210/endocr/bqad041
21154  Nashif SK, Mahr RM, Jena S, Jo S, Nelson AB, Sadowski D, Crawford PA, Puchalska P, Alejandro EU, Gearhart MD, Wernimont SA.  Metformin impairs trophoblast metabolism and differentiation in a dose-dependent manner  Front Cell Dev Biol  2023  11:1167097  PubMed ID: 37250894   DOI: 10.3389/fcell.2023.1167097
21297  Ohyama N, Furugen A, Sawada R, Aoyagi R, Nishimura A, Umazume T, Narumi K, Kobayashi M.  Effects of valproic acid on syncytialization in human placental trophoblast cell lines.  Toxicol Appl Pharmacol  2023  116611  PubMed ID: 37385477   DOI: 10.1016/j.taap.2023.116611

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