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Cell No. : Cell Name
RCB4920 : PSPA  update : 2024/11/13
Comment
Comment from the depositorA preadipocyte clonal line established from porcine subcutaneous tissue of 85-day fetuses. When appropriately induced by the stimulants, fibroblast-like preadipocytes can differentiate into lipid-filled adipocytes.
Terms and conditions1) A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nakajima, et al. Biochem Biophys Res Commun.2003 Sep 26;309(3):702-8) designated by the DEPOSITOR is requested. 3) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
approver's address
×
English
Address
The National Agriculture and Food Research Organization
Institute of Livestock and Grassland Science, NARO, Animal Products Research Division
2, Ikenodai, Tsukuba-Shi, Ibaraki-Ken, 305-0901
Dr.Nakajima, Ikuyo
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor NAKAJIMA, Ikuyo
Originator NAKAJIMA, Ikuyo
Year of deposit 2017
Original cell FPB85
Cloning (depositor) Yes
_Method (depositor) limiting dilution
Animal _pig < Mammals
Genus Sus
Species scrofa
Gender Male
Age at sampling 85-day fetuses
Classification semi-normal
Recombinant non-recombinant
Lifespan finite
Crisis Passage 64 Passage
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_IN46
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM low glucose, pyruvate (Gibco 11885-084)、10%FBS (Life Tech 10099091) DMEM(low glucose) + 10% FBS
Antibiotics 1,000U Penicillin・1mg/mL Streptomycin（Sanko Junyaku Co., Ltd SS420-0001、1:500） Free
Passage method 0.05% Trypsin + 0.01% EDTA 0.05% Trypsin + 0.01% EDTA
Designated culture dish Nunc (EasYFlask,Cell-Culture Treated Multidish)、Sumilon（adhesive cell culture dish）
Culture information Passage ratio 1x10 4 cells/ cm 2 1x10 4 cells/cm 2
SC frequency once/3-4 days Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Images
deposit info
lot info
Relational File deposit infolot info
RCB4920_en.pdf
Reference information Reference 7
User's Publication 0


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Reference
12739  Ikuyo Nakajima, Fumio Nakamura, Koichi Chikuni   Essential role of extracellular type V collagen for porcine adipocyte development   The Japanese Journal of Swine Science  2021  in press   
12738  Satoshi Watanabe, Takayuki Sakurai, Shingo Nakamura, Kazuchika Miyoshi, Masahiro Sato  The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones  Int J Mol Sci  2018  19(4):1075  PubMed ID: 29617297   DOI: 10.3390/ijms19041075
12565  Ikuyo NAKAJIMA  Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development  Japan Agricultural Research Quarterly: JARQ  2015  49:85-90    DOI: 10.6090/jarq.49.85
12737  Masaaki Taniguchi, Ikuyo Nakajima, Koichi Chikuni, Misaki Kojima, Takashi Awata, Satoshi Mikawa  MicroRNA-33b downregulates the differentiation and development of porcine preadipocytes  Mol Biol Rep  2014  41(2):1081-90  PubMed ID: 24398549   DOI: 10.1007/s11033-013-2954-z
12736  T Matsumoto, I Nakajima, T Eguchi-Ogawa, Y Nagamura, N Hamasima, H Uenishi  Changes in gene expression in a porcine preadipocyte cell line during differentiation  Anim Genet  2012  43(5):535-44  PubMed ID: 22497428   DOI: 10.1111/j.1365-2052.2011.02310.x
12735  Sayoko Suzuki, Shin-ichi Ishikawa, Keizou Arihara, Makoto Itoh  Continuous changes in triacylglycerol molecular species composition by fatty acids in porcine adipocytes  Biosci Biotechnol Biochem  2008  72(5):1212-8  PubMed ID: 18460815   DOI: 10.1271/bbb.70674
12564  Ikuyo Nakajima, Susumu Muroya, Koichi Chikuni  Growth arrest by octanoate is required for porcine preadipocyte differentiation  Biochem Biophys Res Commun  2003  309(3):702-8  PubMed ID: 12963048   DOI: 10.1016/j.bbrc.2003.08.057

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