Cell No. : Cell Name
RCB4919 : HeLa/Fucci(CA)5#16
update : 2025/12/16
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | The HeLa cells originally obtained in 1999 from Dr. Mikoshiba’s laboratory at the Institute of Medical Science, The University of Tokyo.All HeLa-related cell lines deposited by the Miyawaki Laboratory, including RCB 2812, RCB 2867, RCB 4676, RCB 4917, and RCB 4919, have been established using this source line. |
| Terms and conditions | 1)If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact : license-contact@innovation-riken.jp 2) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Ando, R. et al., Cell Struct Funct 2023 Jun 30 (2023). |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyawaki, Atsushi
|
| Originator |
Miyawaki, Atsushi
|
| Year of deposit |
2017
|
| Another name |
HeLa/Fucci6#16
|
| Original cell |
HeLa.S3
|
| Cloning (depositor) |
Yes
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
tFucci(CA)5
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A7AU
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM (low glucose) + 10% FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
penicillin/streptomycin
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
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