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Cell No. : Cell Name
RCB4917 : HeLa/Fucci(SA)5#16  update : 2025/01/15
CommentImportant notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositora subclone of HeLa.S3 cells provided by Dr. Mikoshiba in 2009.
Terms and conditions1)If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact : license-contact@innovation-riken.jp 2) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Ando, R. et al., bioRxiv doi: 10.1101/2020.03.30.015156 (2020).
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2017
Another name HeLa/Fucci5#16
Original cell HeLa.S3
Cloning (depositor) Yes
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervical
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene tFucci(SA)5
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7AV
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM (low glucose) + 10% FBS DMEM (low glucose) + 10% FBS
Antibiotics penicillin/streptomycin Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
18122  Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A.   Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression  Cell Struct Funct  2023  Jun 30  PubMed ID: 37394513   DOI: 10.1247/csf.23028

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User's Publication
22125  Nojima H, Kaida A, Matsuya Y, Uo M, Yoshimura RI, Arazi L, Miura M.  DNA damage response in a 2D-culture model by diffusing alpha-emitters radiation therapy (Alpha-DaRT)  Sci Rep  2024  14(1):11468  PubMed ID: 38769339   DOI: 10.1038/s41598-024-62071-6
15096  Tohyama K, Kaida A, Miura M.  Determination of Clonogenic Radiosensitivity of HeLa Cells in Early and Late G1 Phases  Anticancer Res  2022  42(11):5407-5413  PubMed ID: 36288874   DOI: 10.21873/anticanres.16045
13371  Shimono H, Kaida A, Homma H, Nojima H, Onozato Y, Harada H, Miura M.  Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency.  Sci Rep  2020    PubMed ID: 33257719   DOI: 10.1038/s41598-020-77969-0



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