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Cell No. : Cell Name
RCB4901 : NRF3-2(E9408-1)  update : 2024/01/17
CommentHybridoma against NRF3-2 (human) : NRF3 (363~415 aa) antigen.
Comment from the depositorNRF3 (363~415 aa) was expressed on the surface of baculovirus as the gp64 fusion protein according to the previously described procedure (J Atheroscler Thromb. 2002;9:233-242).
Terms and conditions1) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 2) No recipient shall use the research material, their progeny or derivatives, commercially without depositor's prior written approval.
approver's address
3rd Fl., Sangakurenkei-Plaza, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Fax. +81-3-5805-7699
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor HAMAKUBO, Takao
Originator HAMAKUBO, Takao
Year of deposit 2017
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name NS-1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name E9408-1
Subclass IgG2a(Kappa)
Immunogen Gp64 fusion NRF3 (363~415 aa) expressed on the budded baculovirus. 
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1.
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-30 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
deposit info
lot info
Reference information Reference 2
User's Publication 0

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13231  Kobayashi A.  Roles of NRF3 in the Hallmarks of Cancer: Proteasomal Inactivation of Tumor Suppressors.  Cancers (Basel)  2020    PubMed ID: 32962187   DOI: 10.3390/cancers12092681
4041  Chowdhury AMMA, Katoh H, Hatanaka A, Iwanari H, Nakamura N, Hamakubo T, Natsume T, Waku T, Kobayashi A.  Multiple regulatory mechanisms of the biological function of NRF3 (NFE2L3) control cancer cell proliferation  Sci Rep  2017  7:12494  PubMed ID: 28970512   DOI: 10.1038/s41598-017-12675-y

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