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Cell No. : Cell Name
RCB4889 : DP2(D5203-1D8-31) update : 2024/01/17 |
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| Comment | Hybridoma against DP2 (mouse) : prostaglandin receptor DP2 (full length) antigen. | |||
| Comment from the depositor | Full length of mouse Prostaglandin D2 receptor DP2 was expressed on the surface of BaF3 cells according to the previously described procedure（Nagata et al. PLos One 2017 Apr 10;12(4):e0175452. doi:10.1371/journal.pone.0175452. eCollection 2017. ). This hybridoma was generated using the recombinant mouse DP2 expressed BaF3 cells as an immunogen. | |||
| Terms and conditions | 1) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 2) No recipient shall use the research material, their progeny or derivatives, commercially without depositor's prior written approval. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | HAMAKUBO, Takao | ||
| Originator | HAMAKUBO, Takao | |||
| Year of deposit | 2017 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_A6ZE | hybridoma | Fusion_cell_1 name | NS-1 |
| Fusion_cell_1 strain | mouse | |||
| Fusion_cell_2 name | spleen | |||
| Fusion_cell_2 strain | mouse, DP２-null mice (ref.12340) crossed with gp64 transgenic mice (ref.12339). | Fusion method | PEG, HAT selection | Antibody name | D5203-1D8-31 |
| Subclass | IgG2a(Kappa) | |||
| Immunogen | mDP2-expressing BaF3 cells mixed with the wild type of budded Baculovirus (ref.12340). Budded Baculovirus was prepared according to the previously described procedure (ref.12339). | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture medium | (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1. | |||
| Culture method | ||||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10-20 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Antibody subclass | IgG2a(Kappa) | |||
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| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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