Cell No. : Cell Name
RCB4884 : AQP4(E5415B-1H7-79)
update : 2024/01/17
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| Comment | Hybridoma against AQP4 (mouse) : aquaporin (full length) antigen. |
| Comment from the depositor | Full length of mouse aquaporin 4 (ｍAQP4) M23 isoform was expressed on the surface of baculovirus according to the previously described procedure ( J.Immunol,Methods. 30: 322(1-2), 114-117, 2007). This hybridoma was generated using the recombinant budded virus as an immunogen. |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 3) In publishing research results obtained by the use of th eBIOLOGICAL RESOURCE, a citation of the literature ref.(Huang P. et al, BBR 2016,7, 77-83) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
HAMAKUBO, Takao
|
| Originator |
HAMAKUBO, Takao
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| Year of deposit |
2017
|
| Animal |
_mouse x mouse
< Hybrid
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| Tissue |
hybridoma
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| Classification |
hybrid cells
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A6ZA
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hybridoma
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Fusion_cell_1 name |
NS-1
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| Fusion_cell_1 strain |
mouse
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| Fusion_cell_2 name |
spleen
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| Fusion_cell_2 strain |
mouse, gp64 transgenic mice with an AQP4 knockout genetic background
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|
Fusion method |
PEG, HAT selection
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Antibody name |
E5415B-1H7-79
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| Subclass |
IgG2a(Kappa)
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| Immunogen |
Full length of ｍAQP4 M23 isoform expressed on the budded baculovirus.
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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(RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1.
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Antibody subclass |
|
IgG2a(Kappa)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
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4035
Huang P, Takai Y, Kusano-Arai O, Ramadhanti J, Iwanari H, Miyauchi T, Sakihama T, Han JY, Aoki M, Hamakubo T, Fujihara K, Yasui M, Abe Y.
The binding property of a monoclonal antibody against the extracellular domains of aquaporin-4 directs aquaporin-4 toward endocytosis
Biochem Biophys Rep
2016
7:77-83
PubMed ID: 28955892
DOI: 10.1016/j.bbrep.2016.05.017
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4036
Kurosawa K, Misu T, Takai Y, Sato DK, Takahashi T, Abe Y, Iwanari H, Ogawa R, Nakashima I, Fujihara K, Hamakubo T, Yasui M, Aoki M.
Severely exacerbated neuromyelitis optica rat model with extensive astrocytopathy by high affinity anti-aquaporin-4 monoclonal antibody
Acta Neuropathol Commun
2015
3:82
PubMed ID: 26637322
DOI: 10.1186/s40478-015-0259-2
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