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Cell No. : Cell Name
RCB4883 : AQP4(E5415A-1H6-68)  update : 2024/03/15
CommentHybridoma against AQP4 (mouse) : aquaporin (full length) antigen.
Comment from the depositorFull length of mouse aquaporin 4 (mAQP4) M23 isoform was expressed on the surface of baculovirus according to the previously described procedure ( J.Immunol,Methods. 30: 322(1-2), 114-117, 2007). This hybridoma was generated using the recombinant budded virus as an immunogen.
Terms and conditions1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.(Huang P. et al, BBR 2016,7, 77-83) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor HAMAKUBO, Takao
Originator HAMAKUBO, Takao
Year of deposit 2017
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A6YZ
hybridoma Fusion_cell_1 name NS-1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, gp64 transgenic mice with an AQP4 knockout genetic background
Fusion method PEG, HAT selection
Antibody name E5415A-1H6-68
Subclass IgG2a(Kappa)
Immunogen Full length of mAQP4 M23 isoform expressed on the budded baculovirus.
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1.
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
4035  Huang P, Takai Y, Kusano-Arai O, Ramadhanti J, Iwanari H, Miyauchi T, Sakihama T, Han JY, Aoki M, Hamakubo T, Fujihara K, Yasui M, Abe Y.  The binding property of a monoclonal antibody against the extracellular domains of aquaporin-4 directs aquaporin-4 toward endocytosis  Biochem Biophys Rep  2016  7:77-83  PubMed ID: 28955892   DOI: 10.1016/j.bbrep.2016.05.017
4036  Kurosawa K, Misu T, Takai Y, Sato DK, Takahashi T, Abe Y, Iwanari H, Ogawa R, Nakashima I, Fujihara K, Hamakubo T, Yasui M, Aoki M.  Severely exacerbated neuromyelitis optica rat model with extensive astrocytopathy by high affinity anti-aquaporin-4 monoclonal antibody  Acta Neuropathol Commun  2015  3:82  PubMed ID: 26637322   DOI: 10.1186/s40478-015-0259-2

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User's Publication
20663  Hayashi MK, Sato K, Sekino Y.  Neurons Induce Tiled Astrocytes with Branches That Avoid Each Other.  Int J Mol Sci  2022  23(8)  PubMed ID: 35456979   DOI: 10.3390/ijms23084161



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