Cell No. : Cell Name
RCB4872 : AQP4(D15107-3D7-33)
update : 2024/06/17
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| Comment | Hybridoma against AQP4 (human) : aquaporin (full length) antigen. |
| Comment from the depositor | Full length of human aquaporin 4 (hAQP4) M23 isoform was expressed on the surface of baculovirus according to the previously described procedure ( J.Immunol,Methods. 30: 322(1-2), 114-117, 2007). This hybridoma was generated using the recombinant budded virus as an immunogen. |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
HAMAKUBO, Takao
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| Originator |
HAMAKUBO, Takao
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| Year of deposit |
2017
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| Animal |
_mouse x mouse
< Hybrid
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| Tissue |
hybridoma
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| Classification |
hybrid cells
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_A6YQ
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hybridoma
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Fusion_cell_1 name |
NS-1
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| Fusion_cell_1 strain |
mouse
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| Fusion_cell_2 name |
spleen
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| Fusion_cell_2 strain |
mouse, BALB/c (gp64 transgenic mouse)
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|
Fusion method |
PEG, HAT selection
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Antibody name |
D15107-3D7-33
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| Subclass |
IgG2a(Kappa)
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| Immunogen |
Full length of hAQP4 M23 isoform expressed on the budded baculovirus.
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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(RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1.
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Antibody subclass |
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IgG2a(Kappa)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
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| User's Publication |
0
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| Reference |
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4033
Miyazaki-Komine K, Takai Y, Huang P, Kusano-Arai O, Iwanari H, Misu T, Koda K, Mitomo K, Sakihama T, Toyama Y, Fujihara K, Hamakubo T, Yasui M, Abe Y,
High avidity chimeric monoclonal antibodies against the extracellular domains of human aquaporin-4 competing with the neuromyelitis optica autoantibody, NMO-IgG
Br J Pharmacol
2016
173:103-114
PubMed ID: 26398585
DOI: 10.1111/bph.13340
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4032
Miyazaki K, Abe Y, Iwanari H, Suzuki Y, Kikuchi T, Ito T, Kato J, Kusano-Arai O, Takahashi T, Nishiyama S, Ikeshima-Kataoka H, Tsuji S, Arimitsu T, Kato Y, Sakihama T, Toyama Y, Fujihara K, Hamakubo T, Yasui M.
Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4
J Neuroimmunol
2013
260:107-116
PubMed ID: 23746426
DOI: 10.1016/j.jneuroim.2013.03.003
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4034
Ramadhanti J, Huang P, Kusano-Arai O, Iwanari H, Sakihama T, Misu T, Fujihara K, Hamakubo T, Yasui M, Abe Y.
A novel monoclonal antibody against the C-terminal region of aquaporin-4
Monoclon Antib Immunodiagn Immunother
2013
32:270-276
PubMed ID: 23909421
DOI: 10.1089/mab.2013.0007
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12339
Ryoichi Saitoh, Toshihiko Ohtomo, Yoshiki Yamada, Nobuo Kamada, Jun-Ichi Nezu, Naoki Kimura, Shin-Ichi Funahashi, Koh Furugaki, Takeshi Yoshino, Yosuke Kawase, Atsuhiko Kato, Otoya Ueda, Kou-Ichi Jishage, Masami Suzuki, Rie Fukuda, Masahiro Arai, Hiroko Iwanari, Kazuaki Takahashi, Toshiko Sakihama, Iwao Ohizumi, Tatsuhiko Kodama, Masayuki Tsuchiya, Takao Hamakubo
Viral envelope protein gp64 transgenic mouse facilitates the generation of monoclonal antibodies against exogenous membrane proteins displayed on baculovirus
J Immunol Methods
2007
322(1-2):104-17
PubMed ID: 17374538
DOI: 10.1016/j.jim.2007.02.005
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