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Cell No. : Cell Name
RCB4722 : eIF2ak(4KO+GCN2)  update : 2024/11/11
Comment
Comment from the depositorThis is an eIF2ak(4KO) RCB4718 MEF cell line in which flag-tagged mouse GCN2 was reintroduced by retrovirus.
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior MTA on use of it from the DEPOSITOR. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication: (Sci Rep.2016,6:32886) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RemarksCRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
approver's address
×
English
Address
Tokushima University
Institute of Advanced Medical Sciences, Division of Molecular Biology
3-18-15 Kuramoto Tokushima, 770-8503 Japan
Dr.OYADOMARI Seiichi
Fax. +81-88-633-9492
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0065.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OYADOMARI, Seiichi
Originator OYADOMARI, Seiichi
Year of deposit 2017
Another name Eif2ak 4KO MEF mGCN2-FLAG
Cloning (depositor) No
Animal _mouse < Mammals
Strain name C57BL/6J
Genus Mus
Species Musculus
Gender Unknown
Age at sampling E13.5
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen, Gcn2
5984, 10870 base
Vector SV40 1:pBSSVD2005, pMXs-mGCN2-FLAG-IP
Recombinant virus No
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_A7BL
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM containing 4500mg/L D-glucose containing 2mM L-glutamine, nonessential amino acids, 55μM 2-ME, and 10%FBS DMEM (high glucose, without L-Glutamine, with Sodium pyruvate) +10%FBS + 2mM L-Glutamine + 0.1mM NEAA + 55μM 2-ME
Antibiotics Free
Passage method 0.05% trypsin 0.25% Trypsin
Culture information Passage ratio 1:20 1 : 20 split
SC frequency Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5291  Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S.  Integrated stress response of vertebrates is regulated by four eIF2α kinases.  Sci Rep  2016  6:32886  PubMed ID: 27633668   DOI: 10.1038/srep32886

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