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Cell No. : Cell Name
RCB4701 : A4F5  update : 2024/01/17
Comment
Comment from the depositorHybridoma cells producing a monoclonal antibody against plexin-A4.
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Neuron 2007 53(4):535-47) designated by the DEPOSITOR is requested.
Remarks
approver's address
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English
Address
4-1-1 Ogawa-Higashi, Kodaira,Tokyo 187-8551, Japan
Dr.Suto, Fumikazu
Fax. +81-42-346-1749
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SUTO, Fumikazu
Originator SUTO, Fumikazu
Year of deposit 2017
Another name 1F5F112G2b (mAb-A4F5)
Animal _mouse x hamster < Hybrid
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name lymphocyte
Fusion_cell_1 strain Armenian hamster
Fusion_cell_1 gender Female
Fusion_cell_2 name NS1 (P3/NSI/1-Ag-4-1)
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name mAb-A4F5 (anti-Plexin-A4)
Immunogen Recombinant protein: fusion protein of Plexin-A4 semadomain and Fc domain of human IgG.
Antigen_name Plexin-A4
Antigen_m.w. 200 kDa
Antigen_character membrane protein
Antigen_epitope extracellular domain
Antigen_positive neuron
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture medium DMEM + 10%FBS DMEM (low glucose) + 10%FBS
Culture method
Antibiotics 100 units/ml penicillin, 10μg/ml streptomycin Free
Passage method dilution dilution
Culture information Passage ratio 1 : 5 split 1 : 5 split
SC frequency twice/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO or CELLBANKER Plus Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 2
User's Publication 0


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Reference
17877  Tawarayama H, Yoshida Y, Suto F, Mitchell KJ, Fujisawa H.  Roles of semaphorin-6B and plexin-A2 in lamina-restricted projection of hippocampal mossy fibers  J Neurosci  2010  30(20):7049-60  PubMed ID: 20484647   DOI: 10.1523/JNEUROSCI.0073-10.2010
17127  Suto F, Tsuboi M, Kamiya H, Mizuno H, Kiyama Y, Komai S, Shimizu M, Sanbo M, Yagi T, Hiromi Y, Chédotal A, Mitchell KJ, Manabe T, Fujisawa H.  Interactions between plexin-A2, plexin-A4, and semaphorin 6A control lamina-restricted projection of hippocampal mossy fibers  Neuron  2007  53(4):535-47  PubMed ID: 17296555   DOI: 10.1016/j.neuron.2007.01.028

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