Cell No. : Cell Name
RCB4698 : HT1080/Fucci(SA)5
update : 2025/03/26
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. Application consideration |
| Comment from the depositor | A subline of the HT1080 cell line expressing Fucci(SA)5, a cell cycle marker. |
| Terms and conditions | 1) If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact : license-contact@innovation-riken.jp 2) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Ando, R. et al., Cell Struct Funct 2023 Jun 30 (2023). |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
MIYAWAKI Atsushi, SAKAUE-SAWANO Asako
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| Originator |
MIYAWAKI Atsushi, SAKAUE-SAWANO Asako
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| Year of deposit |
2016
|
| Original cell |
HT1080
|
| Cloning (depositor) |
Yes
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
35 years
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| Tissue |
acetabulum
|
| Tumor |
fibrosarcoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
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| Exogene |
Fucci(SA)
|
| Recombinant virus |
No
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM, 10%FBS
|
DMEM (low glucose) + 10% FBS
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| Antibiotics |
penicillin/streptomycin
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Free
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|
Passage method |
|
0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
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| Freeze medium |
Cell Banker
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
Ok
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| STR(human) |
|
OK
|
| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
|
22958
Toshiyuki Goto, Masayuki Nakano, Sally Danno, Chie Ueda, Asako Sakaue-Sawano, Atsushi Miyawaki, Anna Wrabel, Ichiro Nakahara, Takahito Nishikata, Akira Mizoguchi, Yasuhisa Tamura, Kei Mizuno, Yosky Kataoka, Kazuo Funabiki
Microendoscopy for periodic intravital end-to-end tumor imaging of cancer cells
Cell Rep Methods
2025
May 28:101056
PubMed ID: 40472849
DOI: 10.1016/j.crmeth.2025.101056
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18122
Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A.
Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression
Cell Struct Funct
2023
Jun 30
PubMed ID: 37394513
DOI: 10.1247/csf.23028
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