This cell line stablly expresses EGFP-LC3-mRFP-LC3Δ, an autophagic flux probe. Cellular autophagic flux can be evaluated by calculating the ratio of GFP against RFP fluorescent intensity.
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Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N.
An Autophagic Flux Probe that Releases an Internal Control.Mol Cell
PubMed ID: 27818143