Cell No. : Cell Name
RCB4695 : GFP-LC3-RFP-LC3ΔG HeLa
update : 2023/03/28
Comment from the depositor
This cell line stablly expresses EGFP-LC3-mRFP-LC3Δ, an autophagic flux probe. Cellular autophagic flux can be evaluated by calculating the ratio of GFP against RFP fluorescent intensity.
Terms and conditions
1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR . 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication: Mol.Cell 2016 https://dx.doi.org/10.1016/j.molcel.2016.09.037) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
The University of Tokyo, Graduate School of Medicine Department of Biochemistry and Molecular Biology,Mizushima Lab. 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
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Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N.
An Autophagic Flux Probe that Releases an Internal Control.Mol Cell
PubMed ID: 27818143
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.Cancer Res