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Cell No. : Cell Name
RCB4695 : GFP-LC3-RFP-LC3ΔG HeLa  update : 2024/07/11
Comment
Comment from the depositorThis cell line stablly expresses EGFP-LC3-mRFP-LC3Δ, an autophagic flux probe. Cellular autophagic flux can be evaluated by calculating the ratio of GFP against RFP fluorescent intensity.
Terms and conditions1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR . 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication: Mol.Cell 2016 https://dx.doi.org/10.1016/j.molcel.2016.09.037) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Remarks
approver's address
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English
Address
The University of Tokyo, Graduate School of Medicine
Department of Biochemistry and Molecular Biology,Mizushima Lab.
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
Dr.MIZUSHIMA Noboru
Fax. +81-3-3815-1490
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mizushima, Noboru
Originator Mizushima, Noboru
Year of deposit 2016
Animal _human < Mammals
Strain name Homo
Genus sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene EGFP-LC3-mRFP-LC3ΔG
Vector (RDB14600)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_UP51
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM (D6546, Sigma-Aldrich) + 10% fetal bovine serum (FBS) (172012, Sigma-Aldrich) + L-glutamine DMEM (high glucose) + 10%FBS
Antibiotics Free
Passage method 0.05% Trypsin/EDTA 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage cell No 2x10 5cells/ 6 cm dish
Passage ratio 1 : 8 split
SC frequency Subculture : 3-6 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma Not Tested (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
4003  Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N.  An Autophagic Flux Probe that Releases an Internal Control.  Mol Cell  2016  64:835-849  PubMed ID: 27818143   DOI: 10.1016/j.molcel.2016.09.037
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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