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Cell No. : Cell Name
RCB4695 : GFP-LC3-RFP-LC3ΔG HeLa update : 2025/01/16 |
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| Comment | ||||
| Comment from the depositor | This cell line stablly expresses EGFP-LC3-mRFP-LC3Δ, an autophagic flux probe. Cellular autophagic flux can be evaluated by calculating the ratio of GFP against RFP fluorescent intensity. | |||
| Terms and conditions | 1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR . 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication: Mol.Cell 2016 https://dx.doi.org/10.1016/j.molcel.2016.09.037) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Mizushima, Noboru | ||
| Originator | Mizushima, Noboru | |||
| Year of deposit | 2016 | |||
| Animal | _human < Mammals | |||
| Strain name | Homo | |||
| Genus | sapiens | |||
| Gender | Female | |||
| Tissue | cervix | |||
| Disease name | cervical carcinoma | |||
| Classification | cancer | |||
| Recombinant | recombinant | |||
| Exogene | EGFP-LC3-mRFP-LC3ΔG | |||
| Vector | (RDB14600) | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_UP51 | |||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | DMEM (D6546, Sigma-Aldrich) + 10% fetal bovine serum (FBS) (172012, Sigma-Aldrich) + L-glutamine | DMEM (high glucose) + 10%FBS | ||
| Antibiotics | Free | Passage method | 0.05% Trypsin/EDTA | 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA) | Culture information | Passage cell No | 2x10 5cells/ 6 cm dish |
| Passage ratio | 1 : 8 split | |||
| SC frequency | Subculture : 3-6 times/week | Subculture : 2 times/week | ||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Medium + 10% DMSO | Medium + 10% DMSO | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | Not Tested | |||
| Animal PCR | OK | |||
| STR(human) | OK | |||
| Images | ||||
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| Reference information | Reference | 2 | ||
| User's Publication | 1 | |||
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