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Cell No. : Cell Name
RCB4693 : ChR2-C2C12  update : 2024/02/06
CommentA subline of the murine C2C12 cell line (RCB0987) which stably expresses channelrhodopsin 2-Venus (ChR2-Venus), one of light-gated cation channels derived from a green alga Chlamydomonas reinhardtii, tagged with a fluorescent protein, Venus.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biotechnol Bioeng 2012;109:199-204.) designated by the DEPOSITOR is required. 2) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishizuka, Toru
Originator Ishizuka, Toru
Year of deposit 2016
Animal _mouse < Mammals
Strain name C3H
Tissue muscle
Classification other
Recombinant recombinant
Exogene ChR2, ChR2-Venus
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_A6JR
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
3664  Asano T, Ishizua T, Yawo H.  Optically controlled contraction of photosensitive skeletal muscle cells.  Biotechnol Bioeng  2012  109(1):199-204  PubMed ID: 21809334   DOI: 10.1002/bit.23285

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User's Publication
21388  Maemura D, Le TS, Takahashi M, Matsumura K, Maenosono S.  Optogenetic Calcium Ion Influx in Myoblasts and Myotubes by Near-Infrared Light Using Upconversion Nanoparticles.  ACS Appl Mater Interfaces  2023    PubMed ID: 37652433   DOI: 10.1021/acsami.3c07028
13486  Takayama I, Kondo N, Kalies S, Heisterkamp A, Terakawa M.  Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes  J Biophotonics  2020  13(7):e202000037  PubMed ID: 32250039   DOI: 10.1002/jbio.202000037
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256   DOI: 10.1074/jbc.274.45.32192



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