Cell No. : Cell Name
RCB4693 : ChR2-C2C12
update : 2024/08/14
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| Comment | A subline of the murine C2C12 cell line (RCB0987) which stably expresses channelrhodopsin 2-Venus (ChR2-Venus), one of light-gated cation channels derived from a green alga Chlamydomonas reinhardtii, tagged with a fluorescent protein, Venus. |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biotechnol Bioeng 2012;109:199-204.) designated by the DEPOSITOR is required. 2) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishizuka, Toru
|
| Originator |
Ishizuka, Toru
|
| Year of deposit |
2016
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Tissue |
muscle
|
| Classification |
other
|
| Recombinant |
recombinant
|
| Exogene |
ChR2, ChR2-Venus
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_A6JR
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8-10 split
|
| SC frequency |
|
Subculture : once/2-3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
3
|
| User's Publication |
|
21388
Maemura D, Le TS, Takahashi M, Matsumura K, Maenosono S.
Optogenetic Calcium Ion Influx in Myoblasts and Myotubes by Near-Infrared Light Using Upconversion Nanoparticles.
ACS Appl Mater Interfaces
2023
PubMed ID: 37652433
DOI: 10.1021/acsami.3c07028
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13486
Takayama I, Kondo N, Kalies S, Heisterkamp A, Terakawa M.
Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes
J Biophotonics
2020
13(7):e202000037
PubMed ID: 32250039
DOI: 10.1002/jbio.202000037
|
|
3969
Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.
Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.
J Biol Chem
1999.
274(45):32192-7
PubMed ID: 10542256
DOI: 10.1074/jbc.274.45.32192
|
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