Cell No. : Cell Name RCB4688 : HEK-DsupKD  update : 2024/11/11
Comment
Comment from the depositor			A subline of the HEK293(RCB1637) cells. Expressing shRNA against Dsup in HEK-Dsup celIs. Dsup is a tardigrade-unique DNA protection protein.
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Commun. 2016;7:12808) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	KUNIEDA, Takekazu
		Originator	HASHIMOTO, Takuma
		Year of deposit	2016
		Another name	Dsup-knockdown HEK293 cells
		Original cell	HEK293 (RCB1637)
		Cloning (depositor)	Yes
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Age at sampling	embryo
		Tissue	kidney
		Classification	transformed
		Recombinant	recombinant
		Exogene	Damage suppressor (Dsup) gene, Dsup shRNA, neomycin resistance, puromycin resistance
		Vector	pCXN2KS-Dsup
		Selection	G418 (700μg/ml), puromycin (2μg/ml)
		Recombinant virus	No
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_A7AX
			deposit info	lot info
		Medium	Medium List
		Culture type	Adherent cells	Adherent cells
		Culture medium	DMEM + 10%FBS	DMEM (low glucose) + 10%FBS
		Antibiotics		Free
		Passage method	0.25% trypsin	0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency	twice/week	Subculture : 2 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	CELLBANKER 1 (CB013, Takara)	Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Reference information		Reference	1
		User's Publication	0

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Reference

7612  Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A, Kunieda T.  Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.  Nat Commun  2016  7:12808  PubMed ID: 27649274   DOI: 10.1038/ncomms12808

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