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Cell No. : Cell Name
RCB4688 : HEK-DsupKD  update : 2024/01/17
Comment
Comment from the depositorA subline of the HEK293(RCB1637) cells. Expressing shRNA against Dsup in HEK-Dsup celIs. Dsup is a tardigrade-unique DNA protection protein.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Commun. 2016;7:12808) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KUNIEDA, Takekazu
Originator HASHIMOTO, Takuma
Year of deposit 2016
Another name Dsup-knock down HEK293 cells
Original cell HEK293 (RCB1637)
Cloning (depositor) Yes
Animal _human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene Damage suppressor (Dsup) gene, Dsupsh RNA, neomycin resistance, puromycin resistance
Vector pCXN2KS-Dsup
Selection G418 (700μg/ml), puromycin (2μg/ml)
Recombinant virus No
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7AX
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM + 10%FBS DMEM (low glucose) + 10%FBS
Antibiotics Free
Passage method 0.25% trypsin 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency twice/week Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK (OK)
Reference information Reference 1
User's Publication 0


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Reference
7612  Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A, Kunieda T.  Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.  Nat Commun  2016  7:12808  PubMed ID: 27649274   DOI: 10.1038/ncomms12808

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