Cell No. : Cell Name
RCB4685 : Dgcr14
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | Anti-Dgcr14 antibody production hybridoma cell |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takada, Ichiro
|
| Originator |
Yanaka, Kaori
|
| Year of deposit |
2016
|
| Animal |
_mouse x mouse
< Hybrid
|
| Classification |
hybrid cells
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A7DL
|
|
hybridoma
|
Fusion_cell_1 name |
P3U1
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_1 gender |
Unknown
|
| Fusion_cell_2 name |
lymphocyte
|
| Fusion_cell_2 strain |
mouse, BDF1
|
| Fusion_cell_2 gender |
Female
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|
Fusion method |
50% polyethylene glycol, HAT, ELIZA, Western blotting
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|
Antibody name |
Anti-Dgcr14
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| Subclass |
IgG
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|
Antigen_name |
Dgcr14
|
| Antigen_m.w. |
65kDa
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|
Antigen_positive |
nervous system
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
GIT + 1ng/ml IL6
|
GIT + 1ng/ml mouse IL-6
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| Antibiotics |
|
Free
|
|
Passage method |
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dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
|
CELLBANKER2
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1, IgG2b(weak), IgG3(weak)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
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