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Cell No. : Cell Name
RCB4681 : MS-K  update : 2023/10/16
CommentMurine cell line derived from sarcoma.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) The RECIPIENT agree to use the BIOLOGICAL RESOURSE only for publication of research papers. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biomed Pharmacother 1991;45:1-8.) designated by the DEPOSITOR is required.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.SUGIMOTO Kenkichi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugimoto, Kenkichi
Originator Sugimoto, Kenkichi, Mori, Kazuhiro et al
Year of deposit 2016
Animal _mouse < Mammals
Strain name C3H/HeN
Genus Mus
Species musculus
Age at sampling 8 weeks
Tissue bone marrow
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_A7AN
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% Horse Serum +10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3663  Shirata K, Suzuki T, Yanaihara N, Sugimoto K, Mori KJ.  Establishment of a sarcoma cell line, MS-K, expressing Ki-ras proto-oncogene product from mouse bone marrow stromal cells.  Biomed Pharmacother.  1991  45(1):1-8  PubMed ID: 2043753   DOI: 10.1016/0753-3322(91)90146-k

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