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Cell No. : Cell Name
RCB4680 : MS-5  update : 2024/03/04
CommentMouse stromal cell line which supports hematopoietic cells.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) The RECIPIENT agree to use the BIOLOGICAL RESOURSE only for publication of research papers. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Exp Hematol 1989;17:145-153.) designated by the DEPOSITOR is required.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.SUGIMOTO Kenkichi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sugimoto, Kenkichi
Originator Itoh, Katsuhiko, Mori, Kazuhiro et al
Year of deposit 2016
Animal _mouse < Mammals
Strain name C3H/HeN
Genus Mus
Species musculus
Gender Male
Age at sampling 8 weeks
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2128
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% Horse Serum +10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 8

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3662  Itoh K, Tezuka H, Sakoda H, Konno M, Nagata K, Uchiyama T, Uchino H, Mori KJ.  Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow.  Exp Hematol  1989  17(2):145-53  PubMed ID: 2783573  

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User's Publication
21305  Becker HJ, Ishida R, Wilkinson AC, Kimura T, Lee MSJ, Coban C, Ota Y, Tanaka Y, Roskamp M, Sano T, Tojo A, Kent DG, Yamazaki S.  Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion.  Cell Stem Cell  2023  30(7):987-1000.e8  PubMed ID: 37385251   DOI: 10.1016/j.stem.2023.06.002
12168  Kana Kuronuma, Aya Yokoi, Tomoya Fukuoka, Muneaki Miyata, Akio Maekawa, Satowa Tanaka, Leo Matsubara, Chie Goto, Miki Matsuo, Hao-Wei Han, Mai Tsuruta, Haruka Murata, Hikari Okamoto, Natsumi Hasegawa, Shigetaka Asano, Mitsuhiro Ito  Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4  Heliyon  2020    PubMed ID: 32322728   DOI: 10.1016/j.heliyon.2020.e03743
16928  Banerjee K, Madhyastha H, Sandur V R, N T M, N T, Thiagarajan P.  Anti-inflammatory and wound healing potential of a clove oil emulsion  Colloids Surf B Biointerfaces  2020  193:111102  PubMed ID: 32442923   DOI: 10.1016/j.colsurfb.2020.111102
9901  Akhela Umapathi, Navya P Nagaraju, Harishkumar Madhyastha, Devendra Jain, Sangly P Srinivas, Vincent M Rotello, Hemant Kumar Daima  Highly Efficient and Selective Antimicrobial Isonicotinylhydrazide-Coated Polyoxometalate-Functionalized Silver Nanoparticles  Colloids Surf B Biointerfaces   2019  184:110522  PubMed ID: 31586898   DOI: 10.1016/j.colsurfb.2019.110522
12321  Sharma P, Madhyastha H, Madhyastha R, Nakajima Y, Maruyama M, Verma KS, Verma S, Prasad J, Kothari SL, Gour VS.  An appraisal of cuticular wax of Calotropis procera (Ait.) R. Br.: Extraction, chemical composition, biosafety and application  J Hazard Mater   2019  368:397-403  PubMed ID: 30690392   DOI: 10.1016/j.jhazmat.2019.01.067
12524  Navya PN, Madhyastha H, Madhyastha R, Nakajima Y, Maruyama M, Srinivas SP, Jain D, Amin MH, Bhargava SK, Daima HK.  Single step formation of biocompatible bimetallic alloy nanoparticles of gold and silver using isonicotinylhydrazide  Mater Sci Eng C Mater Biol Appl  2019  96:286-294  PubMed ID: 30606534   DOI: 10.1016/j.msec.2018.11.024
4409  Medha M.Ugru et al  Insight into the composition and surface corona reliant biological behaviour of quercetin engineered nanoparticles  Colloids and Surfaces A: Physicochemical and Engineering Aspects  2018  548, 1-9    DOI: 10.1016/j.colsurfa.2018.03.055
12900  Yamaguchi Y, Madhyastha H, Madhyastha R, Choijookhuu N, Hishikawa Y, Pengjam Y, Nakajima Y, Maruyama M.  Arsenic acid inhibits proliferation of skin fibroblasts, and increases cellular senescence through ROS mediated MST1-FOXO signaling pathway  J Toxicol Sci  2016  41(1):105-13  PubMed ID: 26763397   DOI: 10.2131/jts.41.105

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