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Cell No. : Cell Name
RCB4680 : MS-5  update : 2018/07/06
CommentMouse stromal cell line which supports hematopoietic cells.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) The RECIPIENT agree to use the BIOLOGICAL RESOURSE only for publication of research papers. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Exp Hematol 1989;17:145-153.) designated by the DEPOSITOR is required.
approver's address
Niigata University, Graduate School of Science and Technology
Master’s Program / Doctoral Program, Life Sciences
8050 Ikarashi 2 no-cho, Nishi-ku, Niigata City, Niigata 950-2181 Japan
Dr.Sugimoto, Kenkichi
Fax. +81-25-262-6151
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Sugimoto, Kenkichi
Originator Itoh, Katsuhiko, Mori, Kazuhiro et al
Year of deposit 2016
Animal mouse < Mammals
Strain name C3H/HeN
Genus Mus
Species musculus
Gender Male
Age at sampling 8 weeks
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% HS
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% Horse Serum +10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 4

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3662  Itoh K, Tezuka H, Sakoda H, Konno M, Nagata K, Uchiyama T, Uchino H, Mori KJ.  Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow.  Exp Hematol  1989  17(2):145-53  PubMed ID: 2783573

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User's Publication
12168  Kana Kuronuma, Aya Yokoi, Tomoya Fukuoka, Muneaki Miyata, Akio Maekawa, Satowa Tanaka, Leo Matsubara, Chie Goto, Miki Matsuo, Hao-Wei Han, Mai Tsuruta, Haruka Murata, Hikari Okamoto, Natsumi Hasegawa, Shigetaka Asano, Mitsuhiro Ito  Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4  Heliyon  2020    PubMed ID: 32322728
9901  Akhela Umapathi, Navya P Nagaraju, Harishkumar Madhyastha, Devendra Jain, Sangly P Srinivas, Vincent M Rotello, Hemant Kumar Daima  Highly Efficient and Selective Antimicrobial Isonicotinylhydrazide-Coated Polyoxometalate-Functionalized Silver Nanoparticles  Colloids Surf B Biointerfaces   2019  184:110522  PubMed ID: 31586898
12321  Sharma P, Madhyastha H, Madhyastha R, Nakajima Y, Maruyama M, Verma KS, Verma S, Prasad J, Kothari SL, Gour VS.  An appraisal of cuticular wax of Calotropis procera (Ait.) R. Br.: Extraction, chemical composition, biosafety and application  J Hazard Mater   2019  368:397-403  PubMed ID: 30690392
4409    Insight into the composition and surface corona reliant biological behaviour of quercetin engineered nanoparticles  Colloids and Surfaces A: Physicochemical and Engineering Aspects  2018  548, 1-9 

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