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Cell No. : Cell Name
RCB4679 : HepG2-NIAS  update : 2021/12/23
CommentA subline of Hep G2 cells that can not only rapidly activate hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity but also induce the formation of bile canaliculus-like structures by utilizing a collagen vitrigel membrane as a culture substratum.
Comment from the depositorHep G2 cells (RCB1648) described as a backup of ECA2870 derived from ECACC were conditioned in culture using a DMEM containing 10% heat-inactivated FBS, 20mM HEPES 100U/ml penicillin and 100μg/ml streptomycin. By the use of collagen vitrigel membrane as a culture substratum, hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity were rapidly activated, and also the formation of bile canaliculus-like structures were induced.
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR . 2) The BIOLOGICAL RESOURSES should not used for any commercial purposes. Basically, there is no restriction regarding academic use. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Oshikata- Miyazaki A, Takezawa T (2015) Cytotechnology DOI:10.1007/s10616-015- 9934-1.) .
approver's address
1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
National Agriculture and Food Research Organization
Dr.Takezawa, Toshiaki
Fax. +81-29-838-6028
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takezawa, Toshiaki
Originator Takezawa, Toshiaki
Year of deposit 2016
Original cell Hep G2 (RCB1648)
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 15 years
Tissue liver
Disease name hepatocyte carcinoma
Classification cancer
Recombinant non-recombinant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7DQ
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives DMEM (GIBCO, 11885-084) + 10% heat inactivated FBS (SIGMA F2442) + 20mM HEPES(GIBCO, 15630-080) DMEM (low glucose) + 10% FBS (heat inactivated) + 20mM HEPES
Antibiotics 100U/ml Penicillin in and 100µg/ml Streptomycin (GIBCO, 15140-148) Free
Passage method 0.05% Trypsin-0.53mM EDTA 0.25% trypsin
Culture information Passage ratio 1 : 2 - 8 1 : 4 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HBV) RCB1648 (-)
Virus (HCV) RCB1648 (-)
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 0

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4024  Oshikata-Miyazaki A, Takezawa T.  Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber.  Cytotechnology  2016  68:1801-1811  PubMed ID: 26660096
11987  Dolores López-Terrada, Sau Wai Cheung, Milton J Finegold, Barbara B Knowles  Hep G2 is a hepatoblastoma-derived cell line  Hum Pathol  2009  40(10):1512-5  PubMed ID: 19751877
1155  Knowles, B B, Howe, C C, Aden, D P  Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.  Science  1980  209:497-9  PubMed ID: 6248960
4984  Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.  Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.  Carcinogenesis  1980  1(10):871-5  PubMed ID: 11219860
465  Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.  Nature  1979  282:615-6  PubMed ID: 233137

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