A subline of Hep G2 cells that can not only rapidly activate hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity but also induce the formation of bile canaliculus-like structures by utilizing a collagen vitrigel membrane as a culture substratum.
Comment from the depositor
Hep G2 cells (RCB1648) described as a backup of ECA2870 derived from ECACC were conditioned in culture using a DMEM containing 10% heat-inactivated FBS, 20mM HEPES 100U/ml penicillin and 100μg/ml streptomycin. By the use of collagen vitrigel membrane as a culture substratum, hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity were rapidly activated, and also the formation of bile canaliculus-like structures were induced.
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1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR . 2) The BIOLOGICAL RESOURSES should not used for any commercial purposes. Basically, there is no restriction regarding academic use. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Oshikata- Miyazaki A, Takezawa T (2015) Cytotechnology DOI:10.1007/s10616-015- 9934-1.) .
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Oshikata-Miyazaki A, Takezawa T.
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