Cell No. : Cell Name
RCB4679 : HepG2-NIAS
update : 2024/11/11
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| Comment | A subline of Hep G2 cells that can not only rapidly activate hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity but also induce the formation of bile canaliculus-like structures by utilizing a collagen vitrigel membrane as a culture substratum. |
| Comment from the depositor | Hep G2 cells (RCB1648) described as a backup of ECACC 2870 derived from ECACC were conditioned in culture using a DMEM containing 10% heat-inactivated FBS, 20mM HEPES 100U/ml penicillin and 100μg/ml streptomycin. By the use of collagen vitrigel membrane as a culture substratum, hepatic functions such as albumin secretion, urea synthesis and CYP3A4 activity were rapidly activated, and also the formation of bile canaliculus-like structures were induced. |
| Terms and conditions | 1) The BIOLOGICAL RESOURSES should not used for any commercial purposes. Basically, there is no restriction regarding academic use. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Toxicol Sci. 2022;47(1):39-50) . |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Takezawa, Toshiaki
|
| Originator |
Takezawa, Toshiaki
|
| Year of deposit |
2016
|
| Original cell |
Hep G2 (RCB1648)
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
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| Age at sampling |
15 years
|
| Tissue |
liver
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| Disease name |
hepatocyte carcinoma
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A7DQ
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM (GIBCO, 11885-084) + 10% heat inactivated FBS (SIGMA F2442) + 20mM HEPES(GIBCO, 15630-080)
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DMEM (low glucose) + 10% FBS (heat inactivated) + 20mM HEPES
|
| Antibiotics |
100U/ml Penicillin in and 100µg/ml Streptomycin (GIBCO, 15140-148)
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Free
|
|
Passage method |
0.05% Trypsin-0.53mM EDTA
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0.25% Trypsin
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Culture information
|
Passage ratio |
1 : 2 - 8
|
1 : 4 split
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| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
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| Virus (HBV) |
|
RCB1648 (-)
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| Virus (HCV) |
|
RCB1648 (-)
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| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
7
|
| User's Publication |
1
|
| Reference |
|
19595
Miaki UZU, Toshiaki TAKEZAWA
Novel Cell Culture Technology to Harvest Hepatic Metabolites Accumulated in the Bile Canaliculus-like Structures between HepG2-NIAS Cells Utilizing Crosstalk with TFK-1 Cells
Nano Biomedicine
2023
15(2):75-87
DOI: 10.11344/nano.15.75
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15953
Takezawa T, Uzu M.
HepG2-NIAS cells, a new subline of HepG2 cells that can enhance not only CYP3A4 activity but also expression of drug transporters and form bile canaliculus-like networks by the oxygenation culture via a collagen vitrigel membrane
J Toxicol Sci
2022
47(1):39-50
PubMed ID: 34987140
DOI: 10.2131/jts.47.39
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4024
Oshikata-Miyazaki A, Takezawa T.
Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber.
Cytotechnology
2016
68:1801-1811
PubMed ID: 26660096
DOI: 10.1007/s10616-015-9934-1
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11987
Dolores López-Terrada, Sau Wai Cheung, Milton J Finegold, Barbara B Knowles
Hep G2 is a hepatoblastoma-derived cell line
Hum Pathol
2009
40(10):1512-5
PubMed ID: 19751877
DOI: 10.1016/j.humpath.2009.07.003
|
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1155
Knowles, B B, Howe, C C, Aden, D P
Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.
Science
1980
209:497-9
PubMed ID: 6248960
DOI: 10.1126/science.6248960
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4984
Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.
Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.
Carcinogenesis
1980
1(10):871-5
PubMed ID: 11219860
DOI: 10.1093/carcin/1.10.871
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465
Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B
Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.
Nature
1979
282:615-6
PubMed ID: 233137
DOI: 10.1038/282615a0
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