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RCB4676 : HeLa/Fucci(CA)2  update : 2019/02/27
CommentImportant notice regarding nomenclature of Fucci.
Comment from the depositorA subline of the HeLa cell line expressing FucciCA, a cell cycle marker.
Terms and conditions1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Masahiro Yo, Naoki Komatsu, Toru Hiratsuka, Takako Kogure, Tetsushi Hoshida, Naoki Goshima, Michiyuki Matsuda, Hiroyuki Miyoshi and Atsushi Miyawaki. Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol Cell. 2017;68:626-640. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
License mCherry
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2016
Another name HeLa/Fucci(CA)2 #3
Original cell HeLa.S
Cloning (depositor) Yes
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Case history cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci(CA)2
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM, 10 % FBS DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell Banker Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
10584  Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.  Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.  Mol. Cell  2017  68(3):626-640  PubMed ID: 29107535
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

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