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Cell No. : Cell Name
RCB4676 : HeLa/Fucci(CA)2  update : 2024/02/06
CommentImportant notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositorA subline of the HeLa cell line expressing Fucci(CA)2, a cell cycle marker. Cell line name : HeLa/Fucci(CA)2 #3.
Terms and conditions1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Masahiro Yo, Naoki Komatsu, Toru Hiratsuka, Takako Kogure, Tetsushi Hoshida, Naoki Goshima, Michiyuki Matsuda, Hiroyuki Miyoshi and Atsushi Miyawaki. Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol Cell. 2017;68:626-640. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2016
Another name HeLa/Fucci(CA)2 #3
Original cell HeLa.S3
Cloning (depositor) Yes
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci(CA)2
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_VM98
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM, 10 % FBS DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell Banker Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 2

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10584  Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.  Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.  Mol. Cell  2017  68(3):626-640  PubMed ID: 29107535   DOI: 10.1016/j.molcel.2017.10.001
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
16718  He YR, He S, Kandel ME, Lee YJ, Hu C, Sobh N, Anastasio MA, Popescu G.  Cell Cycle Stage Classification Using Phase Imaging with Computational Specificity  ACS Photonics  2022  9(4):1264-1273  PubMed ID: 35480491   DOI: 10.1021/acsphotonics.1c01779
13371  Shimono H, Kaida A, Homma H, Nojima H, Onozato Y, Harada H, Miura M.  Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency.  Sci Rep  2020    PubMed ID: 33257719   DOI: 10.1038/s41598-020-77969-0

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