Cell No. : Cell Name
RCB4676 : HeLa/Fucci(CA)2
update : 2025/03/17
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | A subline of the HeLa cell line expressing Fucci(CA)2, a cell cycle marker. Cell line name : HeLa/Fucci(CA)2 #3. |
| Terms and conditions | 1)The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 3) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 4) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A.et al., Mol. Cell 68 (3): 626-640.e5 (2017). |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyawaki, Atsushi
|
| Originator |
Miyawaki, Atsushi
|
| Year of deposit |
2016
|
| Another name |
HeLa/Fucci(CA)2 #3
|
| Original cell |
HeLa.S3
|
| Cloning (depositor) |
Yes
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
Fucci(CA)2
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_VM98
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM, 10 % FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Cell Banker
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
2
|
| Reference |
|
10584
Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.
Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.
Mol. Cell
2017
68(3):626-640
PubMed ID: 29107535
DOI: 10.1016/j.molcel.2017.10.001
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
| User's Publication |
|
16718
He YR, He S, Kandel ME, Lee YJ, Hu C, Sobh N, Anastasio MA, Popescu G.
Cell Cycle Stage Classification Using Phase Imaging with Computational Specificity
ACS Photonics
2022
9(4):1264-1273
PubMed ID: 35480491
DOI: 10.1021/acsphotonics.1c01779
|
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13371
Shimono H, Kaida A, Homma H, Nojima H, Onozato Y, Harada H, Miura M.
Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency.
Sci Rep
2020
PubMed ID: 33257719
DOI: 10.1038/s41598-020-77969-0
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