Cell No. : Cell Name
RCB4676 : HeLa/Fucci(CA)2
update : 2024/11/11
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | A subline of the HeLa cell line expressing Fucci(CA)2, a cell cycle marker. Cell line name : HeLa/Fucci(CA)2 #3. |
| Terms and conditions | 1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE，a citation of the following literature is necessary. Asako Sakaue-Sawano, Masahiro Yo, Naoki Komatsu, Toru Hiratsuka, Takako Kogure, Tetsushi Hoshida, Naoki Goshima, Michiyuki Matsuda, Hiroyuki Miyoshi and Atsushi Miyawaki. Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol Cell. 2017;68:626-640. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyawaki, Atsushi
|
| Originator |
Miyawaki, Atsushi
|
| Year of deposit |
2016
|
| Another name |
HeLa/Fucci(CA)2 #3
|
| Original cell |
HeLa.S3
|
| Cloning (depositor) |
Yes
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
Fucci(CA)2
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_VM98
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM, 10 % FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
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| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Cell Banker
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
2
|
| Reference |
|
10584
Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.
Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.
Mol. Cell
2017
68(3):626-640
PubMed ID: 29107535
DOI: 10.1016/j.molcel.2017.10.001
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
| User's Publication |
|
16718
He YR, He S, Kandel ME, Lee YJ, Hu C, Sobh N, Anastasio MA, Popescu G.
Cell Cycle Stage Classification Using Phase Imaging with Computational Specificity
ACS Photonics
2022
9(4):1264-1273
PubMed ID: 35480491
DOI: 10.1021/acsphotonics.1c01779
|
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13371
Shimono H, Kaida A, Homma H, Nojima H, Onozato Y, Harada H, Miura M.
Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency.
Sci Rep
2020
PubMed ID: 33257719
DOI: 10.1038/s41598-020-77969-0
|
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