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Cell No. : Cell Name
RCB4675 : NMuMG/Fucci(CA)2.1 update : 2024/01/25 |
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. | |||
| Comment from the depositor | A subline of the NMuMG cell line expressing Fucci(CA)2.1, a cell cycle marker. Cell line name : NMuMG/Fucci(CA)2.1 #2. | |||
| Terms and conditions | 1)The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKENBRC. 2) ARECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE, a citation of the following literature is necessary. (in preparation) 4)The RECIPIENT agrees to acknowledge the DEPOSITER in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication tot he DEPOSITER. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | MIYAWAKI, Atsushi | ||
| Originator | MIYAWAKI, Atsushi | |||
| Year of deposit | 2016 | |||
| Original cell | NMuMG | |||
| Cloning (depositor) | Yes | |||
| Animal | _mouse < Mammals | |||
| Strain name | NAMRU | |||
| Gender | Female | |||
| Tissue | mammary gland | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | FucciCA | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | DMEM(high), 10 % FBS | DMEM (high glucose) + 10% FBS | ||
| Antibiotics | Penicillin, Streptomycin | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 10-20 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | CELLBANKER | Medium + 10% DMSO | ||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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